Identification of a common developmental pathway for thymic natural killer cells and dendritic cells

被引:92
作者
Márquez, C
Trigueros, C
Franco, JM
Ramiro, AR
Carrasco, YR
López-Botet, M
Toribio, ML [1 ]
机构
[1] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, Madrid 28049, Spain
[2] Hosp Princesa, Serv Inmunol, Madrid, Spain
关键词
D O I
10.1182/blood.V91.8.2760.2760_2760_2771
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Current data support the notion that the thymus is seeded by a yet uncommitted progenitor cell able to generate T cells, B cells, natural killer (NK) cells, and dendritic cells (DCs). We assess in this report the developmental relationship of DCs and NK cells derived from a small subset of CD34(+) human postnatal thymocytes that, like the earliest precursors in the fetal thymus, display low CD33 surface expression. Culture of these isolated CD34(+) CD33(lo) thymic progenitors with a mixture of cytokines, including interleukin-7 (IL-7), IL-1 alpha, IL-6, granulocyte-macrophage colony-stimulating factor, and stem cell factor, results in predominant generation of DCs, However, the addition of IL-2 to the cytokine mixture leads to the simultaneous development of DCs and NK cells. Both developmental pathways progress through a transient population of CD34(+)CD44(bright) CD5(lo/-)CD33(+) large-sized cells, distinct from small-sized T-lineage precursors, that contain bipotential NK/DC progenitors. These data provide evidence of linked pathways of NE cell and DC development from intrathymic precursors and suggest that NK cells and DCs branch off the T lineage through a common intermediate progenitor, (C) 1998 by The American Society of Hematology.
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收藏
页码:2760 / 2771
页数:12
相关论文
共 40 条
[1]   Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells [J].
Aiba, Y ;
Hirayama, F ;
Ogawa, M .
BLOOD, 1997, 89 (11) :4005-4012
[2]   THYMIC DENDRITIC CELLS AND T-CELLS DEVELOP SIMULTANEOUSLY IN THE THYMUS FROM A COMMON PRECURSOR POPULATION [J].
ARDAVIN, C ;
WU, L ;
LI, CL ;
SHORTMAN, K .
NATURE, 1993, 362 (6422) :761-763
[3]   LYMPHOID AND MYELOID DIFFERENTIATION OF FETAL LIVER CD34+ LINEAGE(-) CELLS IN HUMAN THYMIC ORGAN-CULTURE [J].
BARCENA, A ;
GALY, AHM ;
PUNNONEN, J ;
MUENCH, MO ;
SCHOLS, D ;
RONCAROLO, MG ;
DEVRIES, JE ;
SPITS, H .
JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 180 (01) :123-132
[4]   Definition of a natural killer NKR-P1A(+)/CD56(-)/CD16(-) functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12 [J].
Bennett, IM ;
Zatsepina, O ;
Zamai, L ;
Azzoni, L ;
Mikheeva, T ;
Perussia, B .
JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 184 (05) :1845-1856
[5]   Identification of a novel developmental stage marking lineage commitment of progenitor thymocytes [J].
Carlyle, JR ;
Michie, AM ;
Furlonger, C ;
Nakano, T ;
Lenardo, MJ ;
Paige, CJ ;
ZunigaPflucker, JC .
JOURNAL OF EXPERIMENTAL MEDICINE, 1997, 186 (02) :173-182
[6]   RECENT ADVANCES IN THE STUDY OF DENDRITIC CELLS AND FOLLICULAR DENDRITIC CELLS [J].
CAUX, C ;
LIU, YJ ;
BANCHEREAU, J .
IMMUNOLOGY TODAY, 1995, 16 (01) :2-4
[7]   GM-CSF AND TNF-ALPHA COOPERATE IN THE GENERATION OF DENDRITIC LANGERHANS CELLS [J].
CAUX, C ;
DEZUTTERDAMBUYANT, C ;
SCHMITT, D ;
BANCHEREAU, J .
NATURE, 1992, 360 (6401) :258-261
[8]  
CICUTTINI FM, 1993, J IMMUNOL, V134, P3731
[9]  
Civin C I, 1993, J Hematother, V2, P137, DOI 10.1089/scd.1.1993.2.137
[10]   Early alpha beta T cell development in the thymus of normal and genetically altered mice [J].
Fehling, HJ ;
vonBoehmer, H .
CURRENT OPINION IN IMMUNOLOGY, 1997, 9 (02) :263-275