Automated DNA mutation detection using universal conditions direct sequencing: application to ten muscular dystrophy genes

被引:7
作者
Bennett, Richard R. [1 ,2 ]
Schneider, Hal E. [1 ,2 ]
Estrella, Elicia [1 ,2 ]
Burgess, Stephanie [1 ,2 ]
Cheng, Andrew S. [3 ,4 ]
Barrett, Caitlin [1 ,2 ]
Lip, Va [3 ,4 ]
Lai, Poh San [5 ]
Shen, Yiping [3 ,4 ]
Wu, Bai-Lin [3 ,4 ]
Darras, Basil T. [6 ]
Beggs, Alan H. [1 ,2 ,7 ]
Kunkel, Louis M. [1 ,2 ,8 ,9 ]
机构
[1] Childrens Hosp, Program Genom, Manton Ctr Orphan Dis Res, Boston, MA 02115 USA
[2] Childrens Hosp, Div Genet, Manton Ctr Orphan Dis Res, Boston, MA 02115 USA
[3] Childrens Hosp, Dept Lab Med, Boston, MA 02115 USA
[4] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA
[5] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Paediat, Singapore 117595, Singapore
[6] Childrens Hosp, Dept Neurol, Boston, MA 02115 USA
[7] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA
[8] Harvard Univ, Sch Med, Dept Genet, Boston, MA USA
[9] Childrens Hosp, Howard Hughes Med Inst, Boston, MA 02115 USA
来源
BMC GENETICS | 2009年 / 10卷
基金
美国国家卫生研究院;
关键词
DEPENDENT PROBE AMPLIFICATION; RATES;
D O I
10.1186/1471-2156-10-66
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Background: One of the most common and efficient methods for detecting mutations in genes is PCR amplification followed by direct sequencing. Until recently, the process of designing PCR assays has been to focus on individual assay parameters rather than concentrating on matching conditions for a set of assays. Primers for each individual assay were selected based on location and sequence concerns. The two primer sequences were then iteratively adjusted to make the individual assays work properly. This generally resulted in groups of assays with different annealing temperatures that required the use of multiple thermal cyclers or multiple passes in a single thermal cycler making diagnostic testing time-consuming, laborious and expensive. These factors have severely hampered diagnostic testing services, leaving many families without an answer for the exact cause of a familial genetic disease. A search of GeneTests for sequencing analysis of the entire coding sequence for genes that are known to cause muscular dystrophies returns only a small list of laboratories that perform comprehensive gene panels. The hypothesis for the study was that a complete set of universal assays can be designed to amplify and sequence any gene or family of genes using computer aided design tools. If true, this would allow automation and optimization of the mutation detection process resulting in reduced cost and increased throughput. Results: An automated process has been developed for the detection of deletions, duplications/insertions and point mutations in any gene or family of genes and has been applied to ten genes known to bear mutations that cause muscular dystrophy: DMD; CAV3; CAPN3; FKRP; TRIM32; LMNA; SGCA; SGCB; SGCG; SGCD. Using this process, mutations have been found in five DMD patients and four LGMD patients (one in the FKRP gene, one in the CAV3 gene, and two likely causative heterozygous pairs of variations in the CAPN3 gene of two other patients). Methods and assay sequences are reported in this paper. Conclusion: This automated process allows laboratories to discover DNA variations in a short time and at low cost.
引用
收藏
页数:18
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