Nerve growth factor potentiates the oxidative necrosis of striatal cholinergic neurons

被引:22
作者
Park, EC [1 ]
Jou, I [1 ]
Gwag, BJ [1 ]
机构
[1] Ajou Univ, Sch Med, Dept Pharmacol, Suwon 442749, Kyungkido, South Korea
关键词
acetylcholine esterase; apoptosis; BDNF; free radical; necrosis; neuron; NGF;
D O I
10.1097/00001756-199803090-00023
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
WE examined the effects of nerve growth factor (NGF) on free radical neurotoxicity in striatal cell cultures. Following exposure to 30 mu M Fe2+ or 1 mM L-buthionine-[S,R]-sulfoximine (BSO), an inhibitor of gamma-glutamylcysteine synthetase, striatal neurons underwent cell body swelling and then widespread death over the next 24 h. The degeneration was prevented by addition of 100 mu M trolox, an antioxidant. Addition of 100 ng/ml BDNF beginning 12 h before Fe2+ or BSO potentiated necrosis of most striatal neurons after exposure to 10 mu M Fe2+ or 1 mM BSO. In contrast, treatment with 100 ng/ml NGF selectively potentiated the oxidative degeneration of striatal cholinergic neurons. The present findings provide additional evidence that NGF, like other neurotrophins, can potentiate oxidative neuronal cell necrosis.
引用
收藏
页码:687 / 690
页数:4
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