Coupled-enzyme system for the determination of lipase activity

被引：5

作者：

Ben Rejeb, I

Ben Hamida, J

Gargouri, M ^{[1
]}

机构：

[1] Natl Inst Appl Sci & Technol, INSAT, Dept Biol & Chem Engn, Tunis, Tunisia

[2] Fac Sci Tunis, Unite Rech Prot & Lipides, Tunis 1060, Tunisia

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 16期

关键词：

enzyme activity determination; lipase; lipoxygenase; two-enzyme system;

D O I：

10.1023/B:BILE.0000044917.68640.c4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new method for lipase activity determination is described, in which liberation of fatty acids by lipase-catalyzed hydrolysis is coupled to lipoxygenation. The second reaction forms hydroperoxy-fatty acids containing a conjugated double bond that are detected at 234 nm. The method is sensitive, cheap and easy to use when compared to a titration method.

引用

页码：1273 / 1276

页数：4

共 7 条

[1] A peroxidase isoenzyme secreted by turnip (Brassica napus) hairy-root cultures:: inactivation by hydrogen peroxide and application in diagnostic kits [J].