Bacterial community structures in honeybee intestines and their response to two insecticidal proteins

被引:165
作者
Babendreier, Dirk [1 ]
Joller, David [1 ]
Romeis, Joerg [1 ]
Bigler, Franz [1 ]
Widmer, Franco [1 ]
机构
[1] Agroscope Reckenholz Tanikon Res Stn ART, CH-8046 Zurich, Switzerland
关键词
transgenic plants; risk assessment; bacteria; Apis mellifera; T-RFLP; Bt-maize;
D O I
10.1111/j.1574-6941.2006.00249.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In this study, the effects of the Bt-toxin Cry1Ab and a soybean trypsin inhibitor (SBTI) on intestinal bacterial communities of adult honeybees (Apis mellifera) were investigated. It was hypothesized that changes in intestinal bacterial communities of honeybees may represent a sensitive indicator for altered intestinal physiology. Honeybees were fed in a laboratory set-up with maize pollen from the Bt-transgenic cultivar MON810 or from the non-transgenic near isoline. Purified Cry1Ab (0.0014% w/v) and SBTI (0.1% or 1% w/v) represented supplementary treatments. For comparison, free-flying honeybees from two locations in Switzerland were analysed. PCR-amplification of bacterial 16S rRNA gene fragments and terminal restriction fragment length polymorphism analyses revealed a total of 17 distinct terminal restriction fragments (T-RFs), which were highly consistent between laboratory-reared and free-flying honeybees. The T-RFs were affiliated to Alpha-, Beta-, and Gammaproteobacteria, to Firmicutes, and to Bacteriodetes. Neither Bt-maize pollen nor high concentrations of Cry1Ab significantly affected bacterial communities in honeybee intestines. Only the high concentration of SBTI significantly reduced the number of T-RFs detected in honeybee midguts, a concentration that also increases bee mortality. Therefore, total bacterial community structures may not be a sensitive indicator for providing evidence for the impact of insecticidal proteins on honeybees at sublethal levels.
引用
收藏
页码:600 / 610
页数:11
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