Metal particle-enhanced fluorescent immunoassays on metal mirrors

被引：74

作者：

Matveeva, Evgenia G. ^{[1
]}

Gryczynski, Ignacy

Barnett, Anne

Leonenko, Zoya

Lakowicz, Joseph R.

Gryczynski, Zygmunt

机构：

[1] Univ N Texas, Hlth Sci Ctr, Dept Mol Biol & Immunol, Ft Worth, TX 76107 USA

[2] Univ N Texas, Hlth Sci Ctr, Dept Cell Biol & Genet, Ft Worth, TX 76107 USA

[3] Macquarie Univ, Div Informat & Commun Sci, Dept Phys, Opt Microcharacterisat Fac, N Ryde, NSW 2109, Australia

[4] Univ Calgary, Fac Med, Dept Cell Biol & Anat, Calgary, AB T2N 4N1, Canada

[5] Univ Maryland, Sch Med, Dept Biochem & Mol Biol, Ctr Fluorescence Spect, Baltimore, MD 21201 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2007年 / 363卷 / 02期

关键词：

fluorescence immunoassay; metal-enhanced fluorescence; plasmonics; nano-size metal particles; silver island films;

D O I：

10.1016/j.ab.2007.01.030

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：239 / 245

页数：7

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