Barrier function, epidermal differentiation, and human β-defensin 2 expression in tinea corporis

被引:55
作者
Jensen, Jens-Michael
Pfeiffer, Stephan
Akaki, Tatsuya
Schroeder, Jens-Michael
Kleine, Michael
Neumann, Claudia
Proksch, Ehrhardt
Brasch, Jochen
机构
[1] Univ Kiel, Dept Dermatol, D-2300 Kiel, Germany
[2] Univ Kiel, Dept Cent Microscopy, D-2300 Kiel, Germany
[3] Planton GmbH, Kiel, Germany
关键词
D O I
10.1038/sj.jid.5700788
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Tinea corporis is a superficial mycotic infection resulting in substantial epidermal changes. We determined skin barrier function, epidermal differentiation, and human-beta-defensin 2 (hBD-2) protein expression in 10 patients with tinea corporis caused by Trichophyton rubrum (T. rubrum). We found disturbed skin barrier function as shown by a significant increase in transepidermal water loss (TEWL) and specific ultrastructural changes including disturbed formation of extracellular lipid bilayers, lamellar body extrusion, and deposit of clotted material at the stratum granulosum/stratum corneum interface. Epidermal proliferation in tinea increased several fold and accordingly, proliferation and inflammation-associated keratins K6, K16, and K17 were expressed. Expression of basal keratins K5 and K14 increased, whereas differentiation-associated K10 was reduced. Reduction of the cornified envelope proteins involucrin, loricrin, and the S100 protein filaggrin was also seen. Reduced filaggrin expression correlated with reduced skin hydration; protein breakdown products of filaggrin have been shown to be important for water binding. Surprisingly, we found pronounced epidermal protein expression of hBD-2, which may be related to disturbed epidermal differentiation and inflammation. hBD-2 showed a weak, although significant, antifungal activity against T rubrum in the turbidimetric assay and the immunohistological staining was somewhat less pronounced in areas directly underneath fungal hyphae in the stratum corneum. Together, we describe profound changes in skin barrier structure and function, epidermal proliferation, and differentiation including pronounced protein expression of hBD-2 in tinea corporis.
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页码:1720 / 1727
页数:8
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