Sequential removal of monosaccharides from the reducing end of oligosaccharides part IV - End-group determination of oligosaccharides: a gas chromatography-mass spectrometry/mass spectrometry method for distinguishing between all D-aldohexoses and D-ketohexoses

被引：5

作者：

Bendiak, B ^{[1
]}

Fang, TT ^{[1
]}

机构：

[1] Univ Colorado, Hlth Sci Ctr, Dept Cellular & Struct Biol, Denver, CO 80262 USA

来源：

CARBOHYDRATE RESEARCH | 2000年 / 327卷 / 04期

关键词：

carbohydrate; oligosaccharide; end-group determination; gas chromatography-mass spectrometry; monosaccharide analysis;

D O I：

10.1016/S0008-6215(00)00058-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for end-group determination of oligosaccharides is described, which involves conversion of the reducing monosaccharide into a 1-deoxy-1-hydrazinohexitol heptaacetate (aldohexoses) or an epimeric pair of 2-deoxy-2-hydrazinohexitol heptaacetates (2-ketohexoses). Products are linear and unique to each aldohexose or ketohexose. Methods are reported for separation of all stereoisomers of the derivatives on single columns by gas chromatography. Gas chromatography-mass spectrometry/mass spectrometry with electron-impact ionization enabled the 1-deoxy-1-hydrazinohexitol heptaacetates and 2-deoxy-2-hydrazinohexitol heptaacetates to be independently identified in each others presence. Chemical-ionization mass spectrometry/mass spectrometry permitted derivatives to be identified in subpicomolar quantities. The non-acetylated compounds could also be identified as their hydrochlorides by H-1 NMR spectroscopy. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：463 / 481

页数：19

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