Identification of mimotopes of platelet autoantigens associated with autoimmune thrombocytopenic purpura

被引：16

作者：

Gevorkian, G ^{[1
]}

Manoutcharian, K ^{[1
]}

Govezensky, T ^{[1
]}

Cano, JA ^{[1
]}

Dominguez, V ^{[1
]}

Santamaria, H ^{[1
]}

Larralde, C ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 04510, DF, Mexico

来源：

JOURNAL OF AUTOIMMUNITY | 2000年 / 15卷 / 01期

关键词：

autoantibodies; autoantigens; autoimmune thrombocytopenic purpura; phage display peptide library;

D O I：

10.1006/jaut.2000.0389

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

GPIIb/IIIa, the human platelet glycoprotein complex, is the autoantigen most commonly recognized by autoantibodies in autoimmune thrombocytopenic purpura (AITP). Two murine monoclonal antibodies (mAbs), namely Y2/51 and 5B12, directed against gpIIIa and gpIIb/IIIa, respectively, and rabbit anti-human platelet polyclonal antibodies have been used to select AITP-related epitopes from a phage display peptide library expressing random dodecapeptides in the pIII coat protein of M13 phage. The selected phage clones were tested by ELISA for binding to rabbit anti-human platelet antibodies as well as to sera from AITP patients. Seven clones reacted strongly with rabbit anti-human platelet antibodies, and four clones reacted with sera from AITP patients. Some homology between peptide inserts sequences of selected clones and human platelet gpIIIa and gpIb were found. (C) 2000 Academic Press.

引用

页码：33 / 40

页数：8

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