Induction of novel organelle DNA variation and transfer of resistance to frost and Verticillium wilt in Solanum tuberosum through somatic hybridization with 1EBN S-commersonii

被引:12
作者
Bastia, T
Carotenuto, N
Basile, B
Zoina, A
Cardi, T
机构
[1] Res Inst Vegetable & Ornamental Plant Breeding, CNR, IMOF, I-80055 Portici, Italy
[2] Univ Naples Federico II, Dept Agron & Plant Genet, I-80055 Portici, Italy
[3] Univ Naples Federico II, Dept Hort Bot & Plant Pathol, I-80055 Portici, Italy
关键词
chloroplasts; mitochondria; potato; Solanum commersonii; somatic hybrids; stress resistance;
D O I
10.1023/A:1003943704037
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Somatic hybridization can be used to induce genetic variability in plastidial and mitochondrial genomes, and transfer multiple uncloned genes across sexual barriers. Somatic hybrids were produced between a dihaploid clone of the common potato, S. tuberosum subsp. tuberosum, and the wild sexually incongruent diploid species S. commersonii. Fusion products were selected on the basis of callus growth and regeneration in vitro. Genome composition of putative somatic hybrids was determined by flow cytometric analysis of nuclear DNA content, RAPD analysis, and Southern analysis with probes specific to organellar DNA. All regenerated fusion products proved to be hybrids based on RAPD analysis. Seventy per cent of somatic hybrids were (near) tetraploids, 22% (near) hexaploids and 8% (near) octoploids. A high correlation was found between the nuclear DNA content determined by flow cytometry and the number of chloroplasts in stomata guard cell pairs. Somatic hybrids inherited the parental plastids in a random manner. On the contrary, they preferentially inherited the mitochondrial DNA fragments of S. tuberosum. The majority of them had a rearranged mitochondrial genome with fragments from both parents. Hybrids were highly vigorous and morphologically more similar to the cultivated than to the wild parent, produced tubers on long stolons under long photoperiod conditions, showed a high degree of flowering, but did not produce pollen. In addition, somatic hybrids were generally more resistant to frost and Verticillium wilt than the cultivated parent, indicating the introgression of relevant resistance genes from the wild species into the genetic background of S. tuberosum.
引用
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页码:1 / 10
页数:10
相关论文
共 41 条
[1]   MOLECULAR CHARACTERIZATION OF INTER-SPECIFIC AND INTRA-SPECIFIC SOMATIC HYBRIDS OF POTATO USING RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS [J].
BAIRD, E ;
COOPERBLAND, S ;
WAUGH, R ;
DEMAINE, M ;
POWELL, W .
MOLECULAR & GENERAL GENETICS, 1992, 233 (03) :469-475
[2]  
BASTIA T, 1998, PLANT BIOTECHNOL, P105
[3]   Production of new CMS Brassica oleracea by transfer of 'Anand' cytoplasm from B-rapa through protoplast fusion [J].
Cardi, T ;
Earle, ED .
THEORETICAL AND APPLIED GENETICS, 1997, 94 (02) :204-212
[4]   PRODUCTION OF SOMATIC HYBRIDS BETWEEN FROST-TOLERANT SOLANUM-COMMERSONII AND SOLANUM-TUBEROSUM - CHARACTERIZATION OF HYBRID PLANTS [J].
CARDI, T ;
DAMBROSIO, F ;
CONSOLI, D ;
PUITE, KJ ;
RAMULU, KS .
THEORETICAL AND APPLIED GENETICS, 1993, 87 (1-2) :193-200
[5]   PRODUCTION OF SOMATIC HYBRIDS BETWEEN FROST TOLERANT SOLANUM-COMMERSONII AND SOLANUM-TUBEROSUM - PROTOPLAST FUSION, REGENERATION AND ISOZYME ANALYSIS [J].
CARDI, T ;
PUITE, KJ ;
RAMULU, KS ;
DAMBROSIO, F ;
FURSCIANTE, L .
AMERICAN POTATO JOURNAL, 1993, 70 (11) :753-764
[6]  
CARDI T, 2000, BIOTECHNOLOGY AGR FO, V49
[7]   Endosperm balance number manipulation for direct in vivo germplasm introgression to potato from a sexually isolated relative (Solanum commersonii Dun.) [J].
Carputo, D ;
Barone, A ;
Cardi, T ;
Sebastiano, A ;
Frusciante, L ;
Peloquin, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (22) :12013-12017
[8]   CHARACTERISTICS OF COLD-ACCLIMATION AND DEACCLIMATION IN TUBER-BEARING SOLANUM SPECIES [J].
CHEN, HH ;
LI, PH .
PLANT PHYSIOLOGY, 1980, 65 (06) :1146-1148
[9]  
Dellaporta S.L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI DOI 10.1007/BF02712670
[10]   COMPARISON OF 3 DNA FLUOROCHROMES FOR FLOW CYTOMETRIC ESTIMATION OF NUCLEAR-DNA CONTENT IN PLANTS [J].
DOLEZEL, J ;
SGORBATI, S ;
LUCRETTI, S .
PHYSIOLOGIA PLANTARUM, 1992, 85 (04) :625-631