Chitinolytic activity of an endophytic strain of Bacillus cereus

被引：104

作者：

Pleban, S ^{[1
]}

Chernin, L ^{[1
]}

Chet, I ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,FAC AGR,OTTO WARBURG CTR BIOTECHNOL AGR,IL-76100 REHOVOT,ISRAEL

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1997年 / 25卷 / 04期

关键词：

D O I：

10.1046/j.1472-765X.1997.00224.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus cereus strain 65, previously isolated as an endophyte of Sinapis, was shown to produce and excrete a chitinase with an apparent molecular mass of 36 kDa. The enzyme was classified as a chitobiosidase because it was able to cleave diacetylchitobiose (GlcNAc)(2) from the non-reducing end of trimeric chitin derivatives. The chitinase exhibited activity over the pH range 4.5-7.5 and was stable between pH 4.0 and 8.5. The enzyme had an isoelectric point of 6.4. Application of B. cereus 65 directly to soil significantly protected cotton seedlings from root rot disease caused by Rhizoctania solani.

引用

页码：284 / 288

页数：5

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