Phenotypes of IRS-2 deficient mice produced by reproductive technology are stable

被引：6

作者：

Hashimoto, Haruo

Arai, Toshiro

Ohnishi, Yasuyuki

Eto, Tomoo

Ito, Mamoru

Hioki, Kyoji

Suzuki, Ryo

Yamauchi, Toshimasa

Ohsugi, Mitsuru

Saito, Muneo

Ueyama, Yoshito

Tobe, Kazuyuki

Kadowaki, Takashi

Tamaoki, Norikazu

Kosaka, Kinori

机构：

[1] Cent Inst Expt Anim, Anim Resource Ctr, Miyamae Ku, Kanagawa 2160001, Japan

[2] Nippon Vet & Life Sci Univ, Fac Vet Med, Dept Vet Biochem, Musashino, Tokyo 1808602, Japan

[3] Univ Tokyo, Grad Sch Med, Dept Metab Dis, Tokyo 1138655, Japan

[4] Tokai Univ, Sch Med, Dept Pathol, Isehara, Kanagawa 2591193, Japan

来源：

EXPERIMENTAL ANIMALS | 2007年 / 56卷 / 02期

关键词：

adiponectin; embryo transfer; IRS-2 deficient mice;

D O I：

10.1538/expanim.56.149

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We studied the impact of "IVF - ET" on the glucose tolerance test (G TT), insulin tolerance test (ITT) and adiponectin to investigate differences in the phenotypes of B6J-lrs2(-/-) mice. The B6J-lrs2(-/-) mice (KO-Nat group) were prepared by natural mating. Other mice were produced by IVF-ET used ICR strain recipients and surrogate mothers (KO-IVF group). Measurement of body weight, GTT, ITT and blood sampling were performed at the ages of 6, 14 and 24 weeks after birth. Body weights, impaired glucose tolerance, insulin resistance and plasma adiponectin concentrations did not differ for each gender between the KO-IVF and KO-Nat groups. Therefore, we concluded that phenotypes of lrs2(-/-) mice produced by reproductive technology are stable.

引用

页码：149 / 154

页数：6

共 23 条

[1]

[Anonymous], 1959, Nature, DOI DOI 10.1038/1841675B0

[2] PRODUCTION OF TRANSGENIC MICE FROM IN-VITRO FERTILIZED-EGGS CRYOPRESERVED BY ULTRARAPID FREEZING [J].