A plant-produced plague vaccine candidate confers protection to monkeys

被引:70
作者
Mett, Vadim
Lyons, Jarred
Musiychuk, Konstantin
Chichester, Jessica A.
Brasil, Trevor
Couch, Ronald
Sherwood, Robert
Palmer, Gene A.
Streatfield, Stephen J.
Yusibov, Vidadi
机构
[1] Fraunhofer USA, Ctr Mol Biotechnol, Newark, DE 19711 USA
[2] Lovelace Biomed & Environm Res Inst, Albuquerque, NM 87108 USA
关键词
plague vaccine; plant-produced vaccine; Yersinia pestis;
D O I
10.1016/j.vaccine.2007.01.017
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Production of vaccine antigens in plants has received considerable attention over the last decade. However, despite many antigens being expressed in plant systems, and promising efficacy data with rodent models, few vaccine candidates have advanced into studies in non-human primates or human clinical trials. Here, we report on the transient expression of the F1 and LcrV antigens of Yersinia pestis in Nicotiana benthamiana. The antigens were expressed as fusions to the thermostable enzyme of Clostridium thermocellum. When administered to Cynomolgus Macaques the purified plant-produced antigens induced serum IgG and IgA responses specific to F1 and LcrV, and conferred complete protection against lethal challenge with Y. pestis. This study clearly demonstrates the efficacy of a plant-produced plague vaccine candidate in a primate model. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3014 / 3017
页数:4
相关论文
共 9 条
  • [1] Monitoring plasma antibiotic concentrations in Spanish hospitals
    Alvarez-Lerma, F
    Grau, S
    Marín-Casino, M
    Olaechea, P
    Sánchez, M
    Martín, E
    Pujol, M
    [J]. ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA, 2006, 24 (01): : 14 - 19
  • [2] Recombinant V antigen protects mice against pneumonic and bubonic plague caused by F1-capsule-positive and -negative strains of Yersinia pestis
    Anderson, GW
    Leary, SEC
    Williamson, ED
    Titball, RW
    Welkos, SL
    Worsham, PL
    Friedlander, AM
    [J]. INFECTION AND IMMUNITY, 1996, 64 (11) : 4580 - 4585
  • [3] Fraction 1 capsular antigen (F1) purification from Yersinia pestis CO92 and from an Escherichia coli recombinant strain and efficacy against lethal plague challenge
    Andrews, GP
    Heath, DG
    Anderson, GW
    Welkos, SL
    Friedlander, AM
    [J]. INFECTION AND IMMUNITY, 1996, 64 (06) : 2180 - 2187
  • [4] Protection against experimental bubonic and pneumonic plague by a recombinant capsular F1-V antigen fusion protein vaccine
    Heath, DG
    Anderson, GW
    Mauro, JM
    Welkos, SL
    Andrews, GP
    Adamovicz, J
    Friedlander, AM
    [J]. VACCINE, 1998, 16 (11-12) : 1131 - 1137
  • [5] MUSIYCHUK K, IN PRESS INFLUENZA O
  • [6] Protection conferred by recombinant Yersinia pestis antigens produced by a rapid and highly scalable plant expression system
    Santi, L
    Giritch, A
    Roy, CJ
    Marillonnet, S
    Klimyuk, V
    Gleba, Y
    Webb, R
    Arntzen, CJ
    Mason, HS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (04) : 861 - 866
  • [7] A single dose sub-unit vaccine protects against pneumonic plague
    Williamson, ED
    Eley, SM
    Stagg, AJ
    Green, M
    Russell, P
    Titball, RW
    [J]. VACCINE, 2000, 19 (4-5) : 566 - 571
  • [8] Human immune response to a plague vaccine comprising recombinant F1 and V antigens
    Williamson, ED
    Flick-Smith, HC
    LeButt, C
    Rowland, CA
    Jones, SM
    Waters, EL
    Gwyther, RJ
    Miller, J
    Packer, PJ
    Irving, M
    [J]. INFECTION AND IMMUNITY, 2005, 73 (06) : 3598 - 3608
  • [9] A NEW IMPROVED SUBUNIT VACCINE FOR PLAGUE - THE BASIS OF PROTECTION
    WILLIAMSON, ED
    ELEY, SM
    GRIFFIN, KF
    GREEN, M
    RUSSELL, P
    LEARY, SEC
    OYSTON, PCF
    EASTERBROOK, T
    REDDIN, KM
    ROBINSON, A
    TITBALL, RW
    [J]. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 1995, 12 (3-4): : 223 - 230