Isolation of 12 microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Puccinia triticina

被引：54

作者：

Duan, X

Enjalbert, J ^{[1
]}

Vautrin, D

Solignac, M

Giraud, T

机构：

[1] INRA, Unite Pathol Vegetale & Epidemiol, F-78850 Thiverval Grignon, France

[2] Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100094, Peoples R China

[3] CNRS, F-91198 Gif Sur Yvette, France

[4] Univ Paris 11, F-91405 Orsay, France

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 01期

关键词：

basidiomycota; biotrophic fungus; dikaryote; leaf rust; microsatellite enriched library; Puccinia triticina;

D O I：

10.1046/j.1471-8286.2003.00350.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the characterization of 12 polymorphic microsatellite markers in the biotrophic fungus Puccinia triticina, the causal agent of leaf rust on wheat. An enrichment protocol was used to isolate microsatellite loci and polymorphism was explored with 15 European isolates. Significant level of cross-amplification (44% of the loci) was found in P. striiformis.

引用

页码：65 / 67

页数：3

共 5 条

[1] Puccinia recondita causing leaf rust on cultivated wheats, wild wheats, and rye [J].