Osteogenic differentiation of mesenchymal stem cells from dental bud: Role of integrins and cadherins

被引:83
作者
Di Benedetto, Adriana [1 ]
Brunetti, Giacomina [2 ]
Posa, Francesca [1 ]
Ballini, Andrea [3 ]
Grassi, Felice Roberto [3 ]
Colaianni, Graziana [2 ]
Colucci, Silvia [2 ]
Rossi, Enzo [6 ]
Cavalcanti-Adam, Elisabetta A. [4 ,5 ]
Lo Muzio, Lorenzo [1 ]
Grano, Maria [2 ]
Mori, Giorgio [1 ]
机构
[1] Univ Foggia, Sch Med, Dept Clin & Expt Med, Vle L Pinto 1, I-71100 Foggia, Italy
[2] Univ Bari, Dept Basic & Med Sci Neurosci & Sense Organs, Sect Human Anat & Histol, I-70121 Bari, Italy
[3] Univ Bari, Dept Basic & Med Sci Neurosci & Sense Organs, I-70121 Bari, Italy
[4] Heidelberg Univ, Dept Biophys Chem, Inst Phys Chem, Stuttgart, Germany
[5] Max Planck Inst Intelligent Syst, Stuttgart, Germany
[6] Private Practice Oral & Maxillofacial Surg, Florence, Italy
关键词
Mesenchymal stem cells; Dental tissues; Dental bud stem cells; Osteogenic differentiation; Cadherin; Integrin; Regenerative medicine; OSTEOBLAST DIFFERENTIATION; N-CADHERIN; IN-VITRO; MEDIATED ADHESION; BETA-CATENIN; PULP; WNT; EXPRESSION; GROWTH; PROLIFERATION;
D O I
10.1016/j.scr.2015.09.011
中图分类号
Q813 [细胞工程];
学科分类号
100113 [医学细胞生物学];
摘要
Several studies have reported the beneficial effects of mesenchymal stem cells (MSCs) in tissue repair and regeneration. New sources of stem cells in adult organisms are continuously emerging; dental tissues have been identified as a source of postnatal MSCs. Dental bud is the immature precursor of the tooth, is easy to access and we show in this study that it can yield a high number of cells with >= 95% expression of mesenchymal sternness makers and osteogenic capacity. Thus, these cells can be defined as Dental Bud Stem Cells (DBSCs) representing a promising source for bone regeneration of stomatognathic as well as other systems. Cell interactions with the extracellular matrix (ECM) and neighboring cells are critical for tissue morphogenesis and architecture; such interactions are mediated by integrins and cadherins respectively. We characterized DBSCs for the expression of these adhesion receptors and examined their pattern during osteogenic differentiation. Our data indicate that N-cadherin and cadherin-11 were expressed in undifferentiated DBSCs and their expression underwent changes during the osteogenic process (decreasing and increasing respectively), while expression of E-cadherin and P-cadherin was very low in DBSCs and did not change during the differentiation steps. Such expression pattern reflected the mesenchymal origin of DBSCs and confirmed their osteoblast-like features. On the other hand, osteogenic stimulation induced the upregulation of single subunits, alpha V, beta 3, alpha 5, and the formation of integrin receptors alpha 5 beta 1 and alpha V beta 3. DBSCs differentiation toward osteoblastic lineage was enhanced when cells were grown on fibronectin (FN), vitronectin (VTN), and osteopontin (OPN), ECM glycoproteins which contain an integrin-binding sequence, the RGD motif. In addition we established that integrin alpha V beta 3 plays a crucial role during the commitment of MSCs to osteoblast lineage, whereas integrin alpha 5 beta 1 seems to be dispensable. These data suggest that functionalization of biomaterials with such ECM proteins would improve bone reconstruction therapies starting from dental stem cells. (C) 2015 The Authors. Published by Elsevier B.V.
引用
收藏
页码:618 / 628
页数:11
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