Identification of a new HLA-DRB4 allele (DRB4*01033) by PCR-SSP and direct sequencing

被引:4
作者
Palou, E [1 ]
Nogués, N [1 ]
Gil, J [1 ]
Ribera, A [1 ]
机构
[1] Ctr Transfusio & Banc Teixits, Serv Immunohematol, Barcelona 08035, Spain
来源
TISSUE ANTIGENS | 2000年 / 56卷 / 03期
关键词
HLA-DRB4; PCR-SSP; nucleotide sequence;
D O I
10.1034/j.1399-0039.2000.560312.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In this report, we describe the identification of a novel DRB4*01 allele, DRB4*01033, found in two Spanish Caucasian individuals. The new allele was detected during routine HLA typing by an unusual pattern of amplification obtained by polymerase chain reaction using sequence-specific primers (PCR-SSP) that did not match with any of the previously described DRB4 alleles. In order to establish the polymorphism responsible for this pattern exons 2 and 3 of the DRB4 locus were amplified and directly sequenced, The new DRB4*01 allele is identical to DRB4*0103101 except for a single nucleotide substitution in codon 78 (TAC-->TAT). This nucleotide change does not cause an amino acid change as both triplets code for a tyrosine.
引用
收藏
页码:279 / 281
页数:3
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