Cytoplasmic polyadenylation element-like sequences are involved in dendritic targeting of BDNF mRNA in hippocampal neurons

被引：39

作者：

Oe, Souichi ^{[2
]}

Yoneda, Yoshihiro ^{[1
,2
,3
]}

机构：

[1] Osaka Univ, Dept Biochem, Grad Sch Med, Suita, Osaka 5650871, Japan

[2] Grad Sch Frontier Biosci, Biomol Dynam Grp, Suita, Osaka 5650871, Japan

[3] Japan Sci & Technol Corp, CREST, Suita, Osaka 5650871, Japan

来源：

FEBS LETTERS | 2010年 / 584卷 / 15期

关键词：

Brain-derived neurotrophic factor; mRNA targeting; Hippocampal neurons; Cytoplasmic polyadenylation element; LOCAL PROTEIN-SYNTHESIS; SYNAPTIC PLASTICITY; KINASE-II; IDENTIFICATION; CPEB; MAP2;

D O I：

10.1016/j.febslet.2010.06.040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several mRNAs are known to be targeted to dendrites in hippocampal neurons. In this study, we show that brain-derived neurotrophic factor (BDNF) mRNA has two distinct as-acting dendritic targeting elements in the short 3' untranslated region (UTR): a constitutive element and an activity-dependent one. Moreover, deletion of serial cytoplasmic polyadenylation element (CPE)-like sequences in the short 3'UTR suppressed both constitutive and activity-dependent dendritic targeting. In addition to the interaction with cytoplasmic polyadenylation element binding protein-1 (CPEB-1), depolarization enhanced CPEB-1 recruitment to the activity-dependent targeting element. These results suggest that CPE-like sequences are involved in the activity-dependent as well as constitutive dendritic targeting of BDNF mRNA. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3424 / 3430

页数：7

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