Prothymosin α associates with the oncoprotein SET and is involved in chromatin decondensation

被引：53

作者：

Karetsou, Z

Martic, G

Tavoulari, S

Christoforidis, S

Wilm, M

Gruss, C

Papamarcaki, T ^{[1
]}

机构：

[1] Univ Ioannina, Sch Med, Biol Chem Lab, GR-45110 Ioannina, Greece

[2] European Mol Biol Lab, Gene Express Programme, D-69117 Heidelberg, Germany

[3] Univ Konstanz, Dept Biol, D-78457 Constance, Germany

来源：

FEBS LETTERS | 2004年 / 577卷 / 03期

关键词：

prothymosin alpha; CREB-binding protein; oncoprotein SET; chromatin decondensation;

D O I：

10.1016/j.febslet.2004.09.091

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prothymosin alpha (ProTalpha) is a histone H1-binding protein that interacts with the transcription coactivator CREB-binding protein and potentiates transcription. Based on coimmunoprecipitation and mammalian two-hybrid assays, we show here that ProTa forms a complex with the oncoprotein SET. ProTa efficiently decondenses human sperm chromatin, while overexpression of GFP-ProTalpha in mammalian cells results in global chromatin decondensation. These results indicate that decondensation of compacted chromatin fibers is an important step in the mechanism of ProTa function. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：496 / 500

页数：5

共 33 条

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