The effects of pulse methylprednisolone on matrix metalloproteinase and tissue inhibitor of metalloproteinase-1 expression in rheumatoid arthritis

被引:19
作者
Wong, P
Cuello, C
Bertouch, JV
Roberts-Thomson, PJ
Ahern, MJ
Smith, MD
Youssef, PP
机构
[1] Univ New S Wales, Sch Pathol, Inflammat Res Unit, Sydney, NSW 2052, Australia
[2] Prince Wales Hosp, Rheumatol Unit, Sydney, NSW, Australia
[3] Flinders Med Ctr, Dept Clin Immunol, Adelaide, SA, Australia
[4] Flinders Med Ctr, Rheumatol Unit, Adelaide, SA, Australia
[5] Flinders Univ S Australia, Dept Med, Adelaide, SA 5001, Australia
[6] Repatriat Gen Hosp, Adelaide, SA, Australia
关键词
rheumatoid arthritis; matrix metalloproteinases; TIMP; corticosteroids;
D O I
10.1093/rheumatology/39.10.1067
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. To investigate the effects of a 1000 mg i.v. pulse of methylprednisolone succinate (pulse therapy) on the expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in the synovial membrane of the knee in patients with rheumatoid arthritis (RA). Methods. Sequential arthroscopic biopsies of the knee were taken before and 24 h after pulse therapy (11 patients), at disease relapse (three patients) and after retreatment with pulse therapy (one patient). Immunoperoxidase staining for MMP-1 (interstitial collagenase), MMP-3 (stromelysin-1) and TIMP-1 was performed and the immunoreactive staining quantified by colour video image analysis. Results. In the synovial lining layer, MMP-1 and TIMP-1 immunostaining was reduced by a mean of 47% (P = 0.02) and 72% (P = 0.05), respectively, 24 h after pulse methylprednisolone therapy. In the synovial sublining layer, MMP-1 was reduced by a mean of 51% (P = 0.08) and TIMP-1 by a mean of 73% (P = 0.02) 24 h after pulse methylprednisolone therapy. There was no change in MMP-3 staining in the synovial lining or sublining layer. Conclusions. High-dose pulse methylprednisolone therapy is associated with a rapid (within 24 h) and substantial decrease in the expression of MMP-1 and TIMP-1 but not MMP-3 in the synovial membrane in RA.
引用
收藏
页码:1067 / 1073
页数:7
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