The mechanism of Ca2+ release from the SR of permeabilised guinea-pig and rat ureteric smooth muscle

被引：19

作者：

Burdyga, TV

Taggart, MJ

Crichton, C

Smith, GL

Wray, S

机构：

[1] Univ Liverpool, Physiol Lab, Liverpool L69 3BX, Merseyside, England

[2] Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1998年 / 1402卷 / 01期

基金：

英国惠康基金;

关键词：

calcium ion; ureter; ryanodine;

D O I：

10.1016/S0167-4889(97)00149-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent work has indicated that there is a major difference in the Ca(2+) store of smooth muscle from rat and guinea-pig ureter; with the rat store being agonist-sensitive but ryanodine insensitive and the guinea-pig store being ryanodine sensitive but agonist insensitive [Th.V. Burdyga, M.J. Taggart, S. Wray, J. Physiol. 489 (1995) 327-335]. We have therefore examined directly the mechanism of Ca(2+) release from the internal Ca(2+) store (SR). Following permeabilisation with alpha-toxin or beta-escin the SR was Ca(2+)-loaded before application of carbachol or caffeine. Only carbachol evoked a transient contraction in rat ureter. The carbachol-induced contraction was blocked by heparin and cyclopiazonic acid (CPA) but not ryanodine, Only caffeine produced contraction in guinea-pig ureter, and this was blocked by ryanodine. Direct application of IP(3) caused a small transient contraction in rat but not guinea-pig ureter. We conclude that rat ureter possesses only an IP(3) sensitive store while guinea-pig ureter only has a ryanodine sensitive store. (C) 1998 Elsevier Science B.V.

引用

页码：109 / 114

页数：6

共 17 条

[1] Major difference between rat and guinea-pig ureter in the ability of agonists and caffeine to release Ca2+ and influence force [J].