High-speed pectic enzyme fractionation by immobilised metal ion affinity membranes

被引：9

作者：

Camperi, SA ^{[1
]}

Grasselli, M ^{[1
]}

Cascone, O ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Microbiol Ind & Biotechnol, RA-1113 Buenos Aires, DF, Argentina

来源：

BIOSEPARATION | 2000年 / 9卷 / 03期

关键词：

hollow-fibre membranes; IMAC; pectic enzymes; purification;

D O I：

10.1023/A:1008187929132

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilised metal ion affinity polysulfone hollow-fibre membranes, with a high capacity for protein adsorption, were prepared and their utilisation for commercial pectic enzyme fractionation was studied. The pass-through fraction containing pectinlyase is useful for fruit-juice clarification without methanol production on account of pectinesterase being retained by the IDA-Cu2+ membrane.

引用

页码：173 / 177

页数：5

共 15 条

[1]

ALANA A, 1989, APPL ENVIRON MICROB, V55, P1612

[2]

Albersheim P., 1966, METHOD ENZYMOL, P628, DOI DOI 10.1016/0076-6879(66)08113-8

[3] METAL-AFFINITY SEPARATIONS - A NEW DIMENSION IN PROTEIN PROCESSING [J].