High-speed pectic enzyme fractionation by immobilised metal ion affinity membranes

被引:9
作者
Camperi, SA [1 ]
Grasselli, M [1 ]
Cascone, O [1 ]
机构
[1] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Microbiol Ind & Biotechnol, RA-1113 Buenos Aires, DF, Argentina
关键词
hollow-fibre membranes; IMAC; pectic enzymes; purification;
D O I
10.1023/A:1008187929132
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Immobilised metal ion affinity polysulfone hollow-fibre membranes, with a high capacity for protein adsorption, were prepared and their utilisation for commercial pectic enzyme fractionation was studied. The pass-through fraction containing pectinlyase is useful for fruit-juice clarification without methanol production on account of pectinesterase being retained by the IDA-Cu2+ membrane.
引用
收藏
页码:173 / 177
页数:5
相关论文
共 15 条
[1]  
ALANA A, 1989, APPL ENVIRON MICROB, V55, P1612
[2]  
Albersheim P., 1966, METHOD ENZYMOL, P628, DOI DOI 10.1016/0076-6879(66)08113-8
[3]   METAL-AFFINITY SEPARATIONS - A NEW DIMENSION IN PROTEIN PROCESSING [J].
ARNOLD, FH .
BIO-TECHNOLOGY, 1991, 9 (02) :151-156
[4]   INTERACTION OF PROTEINS WITH IMMOBILIZED CU2+ - QUANTITATION OF ADSORPTION CAPACITY, ADSORPTION-ISOTHERMS AND EQUILIBRIUM-CONSTANTS BY FRONTAL ANALYSIS [J].
BELEW, M ;
YIP, TT ;
ANDERSSON, L ;
PORATH, J .
JOURNAL OF CHROMATOGRAPHY, 1987, 403 :197-206
[5]   Study of variables involved in fungal pectic enzyme fractionation by immobilized metal ion affinity chromatography [J].
Camperi, SA ;
Auday, RM ;
delCanizo, AN ;
Cascone, O .
PROCESS BIOCHEMISTRY, 1996, 31 (01) :81-87
[6]   PREDICTION OF THE PERFORMANCE OF PREPARATIVE AFFINITY-CHROMATOGRAPHY [J].
CHASE, HA .
JOURNAL OF CHROMATOGRAPHY, 1984, 297 (AUG) :179-202
[7]  
DELCANIZO AN, 1994, J SCI FOOD AGR, V64, P527
[8]  
KRONER KH, 1992, BIOFORUM, V12, P455
[9]   Module performance of anion-exchange porous hollow-fiber membranes for high-speed protein recovery [J].
Kubota, N ;
Konno, Y ;
Saito, K ;
Sugita, K ;
Watanabe, K ;
Sugo, T .
JOURNAL OF CHROMATOGRAPHY A, 1997, 782 (02) :159-165
[10]  
ROMBOUTS FM, 1980, MICROBIAL ENZYMES BI, V5, P228