Chemosensory proteins of Locusta migratoria

被引：145

作者：

Ban, L

Scaloni, A

Brandazza, A

Angeli, S

Zhang, L

Yan, Y

Pelosi, P

机构：

[1] Univ Pisa, Dipartimento Chim & Biotecnol Agr, I-56124 Pisa, Italy

[2] China Agr Univ, Dept Entomol, Beijing, Peoples R China

[3] CNR, Proteom & Mass Spectrometry Lab, ISPAAM, Naples, Italy

来源：

INSECT MOLECULAR BIOLOGY | 2003年 / 12卷 / 02期

关键词：

chemosensory proteins; pheromone; chemical communication; fluorescence binding assay; Locusta migratoria;

D O I：

10.1046/j.1365-2583.2003.00394.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two different classes of chemosensory proteins (CSPs) in Locusta migratoria have been identified on the basis of the molecular cloning of a series of different cDNAs from the antennae of this insect. Several CSP isoforms have been purified and biochemically characterized from antennal and wing extracts, some of them corresponding to expression products predicted for the identified cDNAs. In wings, the nature of the main endogenous ligand binding to these proteins was determined as oleoamide by a gas chromatography-mass spectrometric approach. One of these isoforms has been expressed in a bacterial system with high yield and used in a fluorescent binding assay. Competitive binding experiments have indicated the presence of long-chain compounds among the best ligands.

引用

收藏

页码：125 / 134

页数：10

相关论文

共 33 条

[1] Purification, structural characterization, cloning and immunocytochemical localization of chemoreception proteins from Schistocerca gregaria [J].

Angeli, S ;

Ceron, F ;

Scaloni, A ;

Monti, M ;

Monteforti, G ;

Minnocci, A ;

Petacchi, R ;

Pelosi, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 262 (03) :745-754

[2] Binding properties of a locust's chemosensory protein [J].

Ban, LP ;

Zhang, L ;

Yan, YH ;

Pelosi, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 293 (01) :50-54

[3] Functional characterization of a new class of odorant-binding proteins in the moth Mamestra brassicae [J].

Bohbot, J ;

Sobrio, F ;

Lucas, P ;

Nagnan-Le Meillour, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 253 (02) :489-494

[4] Revisiting the specificity of Mamestra brassicae and Antheraea polyphemus pheromone-binding proteins with a fluorescence binding assay [J].

Campanacci, V ;

Krieger, J ;

Bette, S ;

Sturgis, JN ;

Lartigue, A ;

Cambillau, C ;

Breer, H ;

Tegoni, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (23) :20078-20084

[5] Chemosensory protein from the moth Mamestra brassicae -: Expression and secondary structure from 1H and 15N NMR [J].

Campanacci, V ;

Mosbah, A ;

Bornet, O ;

Wechselberger, R ;

Jacquin-Joly, E ;

Cambillau, C ;

Darbon, H ;

Tegoni, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (17) :4731-4739

[6] Separation, characterization and sexual heterogeneity of multiple putative odorant-binding proteins in the honeybee Apis mellifera L. (Hymenoptera: Apidea) [J].

Danty, E ;

Arnold, G ;

Huet, JC ;

Huet, D ;

Masson, C ;

Pernollet, JC .

CHEMICAL SENSES, 1998, 23 (01) :83-91

[7] Functional and expression pattern analysis of chemosensory proteins expressed in antennae and pheromonal gland of Mamestra brassicae [J].

Jacquin-Joly, E ;

Vogt, RG ;

François, MC ;

Nagnan-Le Meillour, P .

CHEMICAL SENSES, 2001, 26 (07) :833-844

[8]

Kitabayashi AN, 1998, INSECT BIOCHEM MOLEC, V28, P785, DOI 10.1016/S0965-1748(98)00058-7

[9] X-ray structure and ligand binding study of a moth chemosensory protein [J].

Lartigue, A ;

Campanacci, V ;

Roussel, A ;

Larsson, AM ;

Jones, TA ;

Tegoni, M ;

Cambillau, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (35) :32094-32098

[10] Disulfide structure of the pheromone binding protein from the silkworm moth, Bombyx mori [J].

Leal, WS ;

Nikonova, L ;

Peng, GH .

FEBS LETTERS, 1999, 464 (1-2) :85-90

← 1 2 3 4 →