Functional expression of pharaonis phoborhodopsin in Escherichia coli

被引：117

作者：

Shimono, K ^{[1
]}

Iwamoto, M ^{[1
]}

Sumi, M ^{[1
]}

Kamo, N ^{[1
]}

机构：

[1] Hokkaido Univ, Fac Pharmaceut Sci, Biophys Chem Lab, Sapporo, Hokkaido 060, Japan

来源：

FEBS LETTERS | 1997年 / 420卷 / 01期

关键词：

pharaonis phoborhodopsin (ppR); pharaonis sensory rhodopsin II (psRII); eubacterial expression; photocycle; flash-photolysis;

D O I：

10.1016/S0014-5793(97)01487-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pharaonis phoborhodopsin, the photoreceptor of the negative phototaxis of archaebacterial. Natronobacterium pharaonis, was functionally expressed in the heterologous system of Escherichia coli. Flash-photolysis on a millisecond time scale indicated that the photochemical properties of ppR expressed in E. coli were the same as those of the native ppR in N. pharaonis. Wt concluded that the integral membrane protein ppR is correctly folded in vivo in the eubacterial E, coli membrane. (C) 1997 Federation of European Biochemical Societies.

引用

页码：54 / 56

页数：3

共 25 条

[1] BIVIN DB, 1986, J GEN MICROBIOL, V132, P2167
[2] GENOME MAPPING IN HALOBACTERIA
CHARLEBOIS, RL
HOFMAN, JD
SCHALKWYK, LC
LAM, WL
DOOLITTLE, WF
[J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1989, 35 (01) : 21 - 29
[3] PHOTOCYCLE OF PHOBORHODOPSIN FROM HALOALKALIPHILIC BACTERIUM (NATRONOBACTERIUM-PHARAONIS) STUDIED BY LOW-TEMPERATURE SPECTROPHOTOMETRY
HIRAYAMA, J
IMAMOTO, Y
SHICHIDA, Y
KAMO, N
TOMIOKA, H
YOSHIZAWA, T
[J]. BIOCHEMISTRY, 1992, 31 (07) : 2093 - 2098
[4] SHAPE OF THE CHROMOPHORE BINDING-SITE IN PHARAONIS PHOBORHODOPSIN FROM A STUDY USING RETINAL ANALOGS
HIRAYAMA, J
IMAMOTO, Y
SHICHIDA, Y
YOSHIZAWA, T
ASATO, AE
LIU, RSH
KAMO, N
[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1994, 60 (04) : 388 - 393
[5] REASON FOR THE LACK OF LIGHT-DARK ADAPTATION IN PHARAONIS PHOBORHODOPSIN - RECONSTITUTION WITH 13-CIS-RETINAL
HIRAYMA, J
KAMO, N
IMAMOTO, Y
SHICHIDA, Y
YOSHIZAWA, T
[J]. FEBS LETTERS, 1995, 364 (02): : 168 - 170
[6] CHROMOPHORE CONFIGURATION OF PHARAONIS PHOBORHODOPSIN AND ITS ISOMERIZATION ON PHOTON-ABSORPTION
IMAMOTO, Y
SHICHIDA, Y
HIRAYAMA, J
TOMIOKA, H
KAMO, N
YOSHIZAWA, T
[J]. BIOCHEMISTRY, 1992, 31 (09) : 2523 - 2528
[7] PHOTOREACTION CYCLE OF PHOBORHODOPSIN STUDIED BY LOW-TEMPERATURE SPECTROPHOTOMETRY
IMAMOTO, Y
SHICHIDA, Y
YOSHIZAWA, T
TOMIOKA, H
TAKAHASHI, T
FUJIKAWA, K
KAMO, N
KOBATAKE, Y
[J]. BIOCHEMISTRY, 1991, 30 (30) : 7416 - 7424
[8] STRUCTURE-FUNCTION STUDIES ON BACTERIORHODOPSIN .11. EXPRESSION OF THE ARCHAEBACTERIAL BACTERIO-OPSIN GENE WITH AND WITHOUT SIGNAL SEQUENCES IN ESCHERICHIA-COLI - THE EXPRESSED PROTEINS ARE LOCATED IN THE MEMBRANE BUT BIND RETINAL POORLY
KARNIK, S
DOI, T
MOLDAY, R
KHORANA, HG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (22) : 8955 - 8959
[9] HALORHODOPSIN - A LIGHT-DRIVEN CHLORIDE-ION PUMP
LANYI, JK
[J]. ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY, 1986, 15 : 11 - 28
[10] SIGNAL FORMATION IN THE HALOBACTERIAL PHOTOPHOBIC RESPONSE MEDIATED BY A 4TH RETINAL PROTEIN (P480)
MARWAN, W
OESTERHELT, D
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1987, 195 (02) : 333 - 342

← 1 2 3 →