An overlooked DNA source for non-invasive genetic analysis in birds

被引:172
作者
Horváth, MB
Martínez-Cruz, B
Negro, JJ
Kalmár, L
Godoy, JA
机构
[1] Eotvos Lorand Univ, Dept Ethol, H-1117 Budapest, Hungary
[2] CSIC, Estac Biol Donana, Seville 41013, Spain
[3] Natl Med Ctr, Inst Haematol & Immunol, H-1113 Budapest, Hungary
关键词
D O I
10.1111/j.0908-8857.2005.03370.x
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Non-invasive sampling is a useful tool for genetic analyses of endangered and/or elusive species, but it is often inapplicable due to the low quality and quantity of the DNA obtained. In this study we show that the blood clot located in the superior umbilicus of the feather shaft is a better source of DNA than the previously used tip samples from moulted feathers. We found that feather clots from museum specimens provided results nearly as good as footpad and better than those from the more commonly used museum skin snips. Feather clots proved to be a good source of DNA for genetic analysis that will significantly facilitate genetic monitoring of wild bird populations.
引用
收藏
页码:84 / 88
页数:5
相关论文
共 20 条
[1]   DNA TYPING OF MUSEUM BIRDS [J].
ELLEGREN, H .
NATURE, 1991, 354 (6349) :113-113
[2]   A simple and universal method for molecular sexing of non-ratite birds [J].
Fridolfsson, AK ;
Ellegren, H .
JOURNAL OF AVIAN BIOLOGY, 1999, 30 (01) :116-121
[3]  
GAUTSCHI B, 2001, THESIS U ZURICH ZURI
[4]   Phylogeography, genetic structure and diversity in the endangered bearded vulture (Gypaetus barbatus, L.) as revealed by mitochondrial DNA [J].
Godoy, JA ;
Negro, JJ ;
Hiraldo, F ;
Donázar, JA .
MOLECULAR ECOLOGY, 2004, 13 (02) :371-390
[5]  
HODGES RD, 1974, HIST FOWL
[6]   Population genetics after fragmentation:: the case of the endangered Spanish imperial eagle (Aquila adalberti) [J].
Martínez-Cruz, B ;
Godoy, JA ;
Negro, JJ .
MOLECULAR ECOLOGY, 2004, 13 (08) :2243-2255
[7]   Eighteen polymorphic microsatellite markers for the highly endangered Spanish imperial eagle (Aquila adalberti) and related species [J].
Martínez-Cruz, B ;
David, VA ;
Godoy, JA ;
Negro, JJ ;
O'Brien, SJ ;
Johnson, WE .
MOLECULAR ECOLOGY NOTES, 2002, 2 (03) :323-326
[8]  
MORIN PA, 1994, J SCI SOC THAILAND, V20, P31
[9]   Quantitative polymerase chain reaction analysis of DNA from noninvasive samples for accurate microsatellite genotyping of wild chimpanzees (Pan troglodytes verus) [J].
Morin, PA ;
Chambers, KE ;
Boesch, C ;
Vigilant, L .
MOLECULAR ECOLOGY, 2001, 10 (07) :1835-1844
[10]   Microsatellite variation and microevolution in the critically endangered San Clemente Island loggerhead shrike (Lanius ludovicianus mearnsi) [J].
Mundy, NI ;
Winchell, CS ;
Burr, T ;
Woodruff, DS .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1997, 264 (1383) :869-875