A modified fluorometric method to quantify the concentration effect (pI50) of photosystem II-inhibiting herbicides

被引：6

作者：

Hiraki, M

Vredenberg, WJ

van Rensen, JJS

Wakabayashi, K

机构：

[1] Univ Wageningen & Res Ctr, Lab Plant Physiol, NL-6703 BD Wageningen, Netherlands

[2] Tamagawa Univ, Grad Sch Agr Sci, Machida, Tokyo 1948610, Japan

来源：

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 2004年 / 80卷 / 03期

关键词：

chlorophyll; chloroplasts; fluorescence induction; herbicides; method; photosystern II;

D O I：

10.1016/j.pestbp.2004.07.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chlorophyll fluorescence induction curves of isolated thylakoids were measured in the absence and in the presence of various concentrations of photosystem II-inhibiting herbicides. A mathematical program was applied to simulate the curves. Based on these simulated curves a new method is developed to determine the concentration effect (pI(50)) of the herbicides. The results of the new method correspond well with reported values in the literature. The method is very convenient and time-saving. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：183 / 191

页数：9

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