PCR-based molecular markers for the fragrance gene in rice (Oryza sativa. L.)

被引：43

作者：

Garland, S ^{[1
]}

Lewin, L

Blakeney, A

Reinke, R

Henry, R

机构：

[1] So Cross Univ, Ctr Plant Conservat Genet, Lismore, NSW 2480, Australia

[2] NSW Agr, Yanco Agr Inst, Yanco, NSW 2703, Australia

[3] BRI Australian Ltd, Grains Proc, N Ryde, NSW 2113, Australia

来源：

THEORETICAL AND APPLIED GENETICS | 2000年 / 101卷 / 03期

关键词：

fragrance; rice; PCR marker; Oryza sativa;

D O I：

10.1007/s001220051492

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The genomic DNA clone RG28, linked to the major fragrance gene of rice (fgr), was assessed for polymorphism in order to produce a PCR-based marker for fragrance. A small mono-nucleotide repeat, that was polymorphic between a pair of fragrant and non-fragrant cultivars, was identified and developed into a co-dominant PCR-based marker. The polymorphism-information-content determinations for three microsatellite markers, that have been genetically mapped near RG28, are also presented. These PCR-based markers will be highly useful in distinguishing fragrance-producing alleles from non-fragrance-producing alleles at the fgr locus.

引用

页码：364 / 371

页数：8

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