Expression of the CB1 cannabinoid receptor in macrophage-like cells from brain tissue: immunochemical characterization by fusion protein antibodies

被引：47

作者：

Sinha, D

Bonner, TI

Bhat, NR

Matsuda, LA

机构：

[1] Med Univ S Carolina, Dept Psychiat & Behav Sci, Charleston, SC 29425 USA

[2] NIMH, Genet Sect, NIH, Bethesda, MD 20892 USA

[3] Med Univ S Carolina, Dept Neurol, Charleston, SC 29425 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1998年 / 82卷 / 01期

关键词：

Marijuana; Cannabis; microglial G protein coupled receptors;

D O I：

10.1016/S0165-5728(97)00181-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Antibodies designed to recognize a 74 amino acid sequence of the N- or C-terminal domain of the rat CBI cannabinoid receptor detected a 58 kDa protein in immunoblots of brain and various cells known to express the CB1 cannabinoid receptor. A human B-lymphoblastoid cell line and macrophage-like cells from neonatal rat brain were also positive for CB1 receptor-like immunoreactivity. Immunocytochemical analysis performed with isolated Fab fragments showed surface staining in NG108-15 cells and brain macrophage like cells which also express MHC class II antigens. The data suggest a plausible role for CB1 receptors in the immune function of brain. (C) 1998 Elsevier Science B.V.

引用

页码：13 / 21

页数：9

共 35 条

[1] THE EXPRESSION OF MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE IN OLIGODENDROCYTES IS DEVELOPMENTALLY-REGULATED [J].