The Release 5.1 annotation of Drosophila melanogaster heterochromatin

被引：150

作者：

Smith, Christopher D.

Shu, ShengQiang

Mungall, Christopher J.

Karpen, Gary H. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Dept Genome & Computat Biol, Drosophila Heterochromatin Genome Project, Berkeley, CA 94720 USA

[2] San Francisco State Univ, Dept Biol, San Francisco, CA 94132 USA

[3] Univ Calif Berkeley, Lawrence Berkeley Lab, Natl Ctr Biomed Ontol, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

SCIENCE | 2007年 / 316卷 / 5831期

关键词：

D O I：

10.1126/science.1139815

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The repetitive DNA that constitutes most of the heterochromatic regions of metazoan genomes has hindered the comprehensive analysis of gene content and other functions. We have generated a detailed computational and manual annotation of 24 megabases of heterochromatic sequence in the Release 5 Drosophila melanogaster genome sequence. The heterochromatin contains a minimum of 230 to 254 protein-coding genes, which are conserved in other Drosophilids and more diverged species, as well as 32 pseudogenes and 13 noncoding RNAs. Improved methods revealed that more than 77% of this heterochromatin sequence, including introns and intergenic regions, is composed of fragmented and nested transposable elements and other repeated DNAs. Drosophila heterochromatin contains "islands" of highly conserved genes embedded in these "oceans" of complex repeats, which may require special expression and splicing mechanisms.

引用

页码：1586 / 1591

页数：6

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