Ischemic heart failure enhances endogenous myocardial apelin and APJ receptor expression

被引：102

作者：

Atluri, Pavan ^{[1
]}

Morine, Kevin J. ^{[1
]}

Liao, George P. ^{[1
]}

Panlilio, Corinna M. ^{[1
]}

Berry, Mark F. ^{[1
]}

Hsu, Vivian M. ^{[1
]}

Hiesinger, William ^{[1
]}

Cohen, Jeffrey E. ^{[1
]}

Woo, Y. Joseph ^{[1
]}

机构：

[1] Univ Penn, Dept Surg, Div Cardiothorac Surg, Philadelphia, PA 19104 USA

来源：

CELLULAR & MOLECULAR BIOLOGY LETTERS | 2007年 / 12卷 / 01期

关键词：

apelin; G protein coupled receptor; APJ; inotrope;

D O I：

10.2478/s11658-006-0058-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apelin interacts with the APJ receptor to enhance inotropy. In heart failure, apelin- APJ coupling may provide a means of enhancing myocardial function. The alterations in apelin and APJ receptor concentrations with ischemic cardiomyopathy are poorly understood. We investigated the compensatory changes in endogenous apelin and APJ levels in the setting of ischemic cardiomyopathy. Male, Lewis rats underwent LAD ligation and progressed into heart failure over 6 weeks. Corresponding animals underwent sham thoracotomy as control. Six weeks after initial surgery, the animals underwent hemodynamic functional analysis in the presence of exogenous apelin- 13 infusion and the hearts were explanted for western blot and enzyme immunoassay analysis. Western blot analysis of myocardial APJ concentration demonstrated increased APJ receptor protein levels with heart failure ( 1890750 +/- 133500 vs. 901600 +/- 143120 intensity units, n= 8, p= 0.00001). Total apelin protein levels increased with ischemic heart failure as demonstrated by enzyme immunoassay ( 12.0 +/- 4.6 vs. 1.0 +/- 1.2 ng/ ml, n= 5, p= 0.006) and western blot ( 1579400 +/- 477733 vs. 943000 +/- 157600 intensity units, n= 10, p= 0.008). Infusion of apelin- 13 significantly enhanced myocardial function in sham and failing hearts. We conclude that total myocardial apelin and APJ receptor levels increase in compensation for ischemic cardiomyopathy.

引用

页码：127 / 138

页数：12

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