Agrobacterium-mediated transformation of monocot and dicot plants using the NCR promoter derived from soybean chlorotic mottle virus

被引：30

作者：

Fukuoka, H

Ogawa, T

Mitsuhara, I

Iwai, T

Isuzugawa, K

Nishizawa, Y

Gotoh, Y

Nishizawa, Y

Tagiri, A

Ugaki, M

Ohshima, M

Yano, H

Murai, N

Niwa, Y

Hibi, T

Ohashi, Y

机构：

[1] Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan

[2] Chugoku Natl Agr Expt Stn, Hiroshima 7218514, Japan

[3] Japan Sci & Technol Corp, CREST, Chiyoda Ku, Tokyo 1010062, Japan

[4] Yamagata Prefectural Hort Expt Stn, Yamagata 9910043, Japan

[5] Hokuriku Natl Agr Exptl Stn, Niigata 9430193, Japan

[6] Louisiana State Univ, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA

[7] LSU Med Ctr, Baton Rouge, LA 70803 USA

[8] Univ Shizuoka, Grad Sch Nutr & Environm Sci, Shizuoka 4228526, Japan

[9] Univ Tokyo, Dept Agr & Environm Biol, Bunkyo Ku, Tokyo 1138657, Japan

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 08期

关键词：

soybean chlorotic mottle virus; Agrobacterium-mediated transformation; NCR promoter; transgenic rice; transgenic tobacco;

D O I：

10.1007/s002990000191

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The NCR promoter (PNCR) from soybean chlorotic mottle virus (SoyCMV) was used to express the selectable marker, neomycin phosphotransferase (nptII) gene, in Agrobacterium-mediated transformation of both monocot (rice) and dicot (tobacco) plants. A multi-cloning site for insertion of a gene of interest into the binary vector pTN is located proximal to the right border region of T-DNA. When chimeric genes under the control of other strong promoters were located in a head-to-head orientation to the PNCR-nptII gene, kanamycin-resistant tobacco shoots were generated more efficiently than when using the original pTN vectors. This suggests that the enhancer-like sequences in the promoters adjacent to PNCR may promote expression of the PNCR-nptII gene.

引用

页码：815 / 820

页数：6

共 23 条

[1] STRUCTURE AND INDUCTION-PATTERN OF A NOVEL PROTEINASE-INHIBITOR CLASS-II GENE OF TOBACCO [J].