Immunodetection of UCP1 in rat thymocytes

被引：20

作者：

Carroll, AM

Haines, LR

Pearson, TW

Brennan, C

Breen, EP

Porter, RK ^{[1
]}

机构：

[1] Univ Dublin Trinity Coll, Dept Biochem, Dublin 2, Ireland

[2] Univ Victoria, Dept Biochem & Microbiol, Victoria, BC, Canada

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2004年 / 32卷

关键词：

brown adipose tissue; cell death-inducing DNA fragmentation factor a-like effector A (Cidea); mitochondria; thymus; uncoupling protein (UCP);

D O I：

10.1042/BST0321066

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thymi were dissected from rats and connective tissue was removed. Mitochondria were purified from isolated thymocytes and immunoblot analysis was performed using an antibody specific for uncoupling protein 1, which detected a 32.5 kDa protein associated with mitochondria from the thymocytes. This implies that rat thymocytes contain uncoupling protein 1.

引用

页码：1066 / 1067

页数：2

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