Fibroblasts and dermal gene therapy: A minireview

被引：16

作者：

Krueger, GG ^{[1
]}

机构：

[1] Univ Utah, Hlth Sci Ctr, Dept Dermatol, Salt Lake City, UT 84132 USA

来源：

HUMAN GENE THERAPY | 2000年 / 11卷 / 16期

关键词：

D O I：

10.1089/104303400750035834

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This review highlights our current understanding of the biology of, survival of, and transgene expression by genetically modified fibroblasts (GMFb) carrying stably integrated transgenes in vivo. Experimental data demonstrate that three elements win enhance expression by and survival of GMFb in vivo: a matrix scaffolding to take the place of the existing dermis, the presence of elements of the extracellular matrix in the construct used to move GMFb to the in vivo setting, and the utilization of immortalized fibroblasts to carry the transgenes. Although moving GMFb to an in vivo setting is an invasive procedure, there are a number of clinical settings where GMFb appear to be the suitable cell for gene therapy.

引用

页码：2289 / 2296

页数：8

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