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Glyceraldehyde-3-phosphate dehydrogenase varies with age in glycolytic muscles of rats

被引：50
作者：
Lowe, DA
Degens, H
Chen, KJD
Alway, SE
机构：
[1] Univ S Florida, Coll Med, Dept Anat, Tampa, FL USA
[2] Univ S Florida, Inst Aging, Tampa, FL USA
来源：
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES | 2000年 / 55卷 / 03期
关键词：
D O I：
10.1093/gerona/55.3.B160
中图分类号：
R592 [老年病学]; C [社会科学总论];
学科分类号：
03 ; 0303 ; 100203 ;
摘要：
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA, protein, and enzyme activity levels in hindlimb muscles of adult and senescent Fischer 344 x Brown Norway rats were investigated. Soleus muscles From adult and senescent rats had similar levels of GAPDH. In contrast, muscles containing a large proportion of glycolytic fibers had lower GAPDH levels in senescent rats relative to these muscles in adult rats; this was observed at both the mRNA and protein levels. These data indicate that skeletal muscle glycolytic capacity of fast muscles is diminished with age and that it may he caused by changes at the level of transcription. Also, because GAPDH mRNA levels change with age in several rat muscles, GAPDH mRNA is not always a proper internal control for mRNA analyses of aging skeletal muscle.
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页码：B160 / B164
页数：5
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