Quantitative analysis of amoA mRNA expression as a new biomarker of ammonia oxidation activities in a complex microbial community

被引：40

作者：

Aoi, Y ^{[1
]}

Masaki, Y ^{[1
]}

Tsuneda, S ^{[1
]}

Hirata, A ^{[1
]}

机构：

[1] Waseda Univ, Dept Chem Engn, Shinjuku Ku, Tokyo 1698555, Japan

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2004年 / 39卷 / 06期

关键词：

ammonia oxidizing bacteria; mRNA; nitrification; real-time reverse transcription-PCR; rRNA; wastewater treatment process;

D O I：

10.1111/j.1472-765X.2004.01585.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To quantitatively analyse the changes to amoA mRNA (ammonia mono-oxygenase encoding mRNA) profiles in response to a change in ammonia oxidation activity in a complex microbial community. Methods and Results: The amoA mRNA levels in both a batch-mode incubation and a continuously fed nitrification reactor were determined by real-time reverse transcription-PCR analysis. The amoA mRNA level changed rapidly in response to the change in environmental conditions which affect ammonia oxidation activity. Conclusion: An increase in amoA mRNA level can be detected within 1-2 h in response to an initiation of cell activity whereas a decrease in amoA mRNA level is detected within 24 h in response to a cessation of activity. Significance and Impact of the Study: amoA mRNA, which shows sensitive response to ammonia oxidation activity, can be used as a biomarker of ammonia oxidation activity in wastewater treatment processes where many bacterial species exist.

引用

页码：477 / 482

页数：6

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