Whisker stimulation increases expression of nerve growth factor- and interleukin-1β-immunoreactivity in the rat somatosensory cortex

Activity-dependent changes in cortical protein expression may mediate long-term physiological processes such as sleep and neural connectivity. In this study we determined the number of nerve growth factor (NGF)- and interleukin-1 beta (IL1 beta)-immunoreactive (IR) cells in the somatosensory cortex (Sctx) in response to 2 h of mystacial whisker stimulation. Manual whisker stimulation for 2 h increased the number of NGF-IR cells within layers II-V in activated Sctx columns, identified by enhanced Fos-IR. IL1 beta-IR neurons increased within layers II-III and V-VI in these activated columns and IR astrocytes increased in layers I, II-III and V as well as the external capsule beneath the activated columns. These whisker-stimulated increases in the Sctx did not occur in the auditory cortex. These data demonstrate that expression of NGF or IL1 beta in Sctx neurons and IL1 beta, in Sctx astrocytes is, in part, afferent input-dependent. (C) 2010 Elsevier B.V. All rights reserved.