Toxicological evaluation of aqueous leaf and berry extracts of Phytolacca dioica L. in male Wistar rats

Despite the widespread use of Phytolacca dioica L in folklore medicine of South Africa, there is dearth of information on its safety/toxicity. The aim of this study was to evaluate the toxicological effect of aqueous leaf and berry extracts of the plant at different dosages for 14 days on the liver and kidney function indices in male Wistar rats. Phytochemical screening indicated that the extracts are rich in phytonutrients including alkaloid, tannin, saponins, phenolics, lectins and flavonoids; while triterpenoids and phlobatanins were absent. The extracts significantly reduced the body and absolute organ weights of the animals at all the dosages investigated. Whereas, significant increase was observed in the serum levels of alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), alanine transaminase (ALT), aspartate transaminase (AST), bilirubin, calcium, creatinine, urea and uric acid; the serum concentration of total protein, albumin and globulin were reduced in the serum following treatment with the extracts. Generally, the effect of the herb appeared to be dosage dependent. This investigation clearly showed that P. dioica can impair normal hepatic and renal functions. This is an indication that the extracts may not be completely safe in male rats when continuously administered for 14 days. (C) 2010 Elsevier Ltd. All rights reserved.