Regulation of myosin phosphatase through phosphorylation of the myosin-binding subunit in platelet activation

Human platelets were found to contain myosin phosphatase consisting of a 38-kD catalytic subunit of protein phosphatase type 1 delta, a 130-kD myosin-binding subunit (NIBS) and a 20-kD subunit, all of which cross-reacted with antibodies against these subunits of smooth muscle myosin phosphatase. Anti-NIBS antibody coimmunoprecipitated RhoA and Rho-kinase of human platelets. Platelets NIBS is a substrate for Rho-kinase and phosphorylation of NIBS decreases the activity of myosin phosphatase. Treatment of intact platelets with 9,11-epithio-11,12-methano-thromboxane A(2) led to a dramatic increase in phosphorylation of MBS and a significant decrease in the activity of myosin phosphatase. These findings suggest a putative mechanism for agonist-induced regulation of myosin phosphatase activity in platelets. (C) 1997 by The American Society of Hematology.