Genotyping assay for the identification of 2009-2010 pandemic and seasonal H1N1 influenza virus reassortants

被引：11

作者：

Ducatez, M. F. ^{[1
]}

Sonnberg, S. ^{[1
]}

Hall, R. J. ^{[2
]}

Peacey, M. ^{[2
]}

Ralston, J. ^{[2
]}

Webby, R. J. ^{[1
]}

Huang, Q. S. ^{[2
]}

机构：

[1] St Jude Childrens Res Hosp, Dept Infect Dis, Memphis, TN 38105 USA

[2] Inst Environm Sci & Res Ltd, Natl Ctr Biosecur & Infect Dis, Upper Hutt, New Zealand

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 168卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

Pandemic influenza; Seasonal influenza; Genotyping PCR; Reassortant; B VIRUSES;

D O I：

10.1016/j.jviromet.2010.04.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

New Zealand identified its first pandemic H1N1 influenza cases in late April 2009, immediately prior to the historical start of the New Zealand influenza season. Both pandemic and oseltamivir-resistant seasonal H1N1 viruses cocirculated in the population for a period of time. Thus, concerns were raised about the possibility of reassortment events between the two strains. An RT-PCR-based genotyping assay was developed so that H1N1 influenza coinfections and reassortants could be detected quickly. The assay differentiated effectively the seasonal and pandemic strains. It also confirmed the identification of the first reported coinfection of pandemic and seasonal H1N1 strains during the 2009 Southern Hemisphere influenza season in New Zealand. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：78 / 81

页数：4

共 18 条

[1] Identification and characterization of a late AH1N2 human reassortant in France during the 2002-2003 influenza season [J].

Al Faress, Shaker ;

Ferraris, Olivier ;

Moules, Vincent ;

Valette, Martine ;

Hay, Alan ;

Lina, Bruno .

VIRUS RESEARCH, 2008, 132 (1-2) :33-41

[2] Amantadine- and oseltamivir-resistant variants of influenza A viruses in Thailand [J].

Bai, Gui-Rong ;

Chittaganpitch, Malinee ;

Kanai, Yuta ;

Li, Yong-Gang ;

Auwanit, Wattana ;

Ikuta, Kazuyoshi ;

Sawanpanyalert, Pathom .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 390 (03) :897-901

[3]

Centers for Disease Control and Prevention (CDC), 2009, MMWR Morb Mortal Wkly Rep, V58, P467

[4] Multiple sequence alignment with the Clustal series of programs [J].

Chenna, R ;

Sugawara, H ;

Koike, T ;

Lopez, R ;

Gibson, TJ ;

Higgins, DG ;

Thompson, JD .

NUCLEIC ACIDS RESEARCH, 2003, 31 (13) :3497-3500

[5]

Eshaghi AliReza, 2009, Int J Infect Dis, V13, pe127, DOI 10.1016/j.ijid.2008.06.024

[6] Dual infections by influenza A/H3N2 and B viruses and by influenza A/H3N2 and A/H1N1 viruses during winter 2007, Corsica Island, France [J].

Falchi, A. ;

Arena, C. ;

Andreoletti, L. ;

Jacques, J. ;

Leveque, N. ;

Blanchon, T. ;

Lina, B. ;

Turbelin, C. ;

Dorleans, Y. ;

Flahault, A. ;

Amoros, J. P. ;

Spadoni, G. ;

Agostini, F. ;

Varesi, L. .

JOURNAL OF CLINICAL VIROLOGY, 2008, 41 (02) :148-151

[7] Evidence for Persistence of and Antiviral Resistance and Reassortment Events in Seasonal Influenza Virus Strains Circulating in Cambodia [J].

Fourment, Mathieu ;

Mardy, Sek ;

Channa, Mey ;

Buchy, Philippe .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (01) :295-297

[8] Occurrence of Mixed Populations of Influenza A Viruses That Can Be Maintained through Transmission in a Single Host and Potential for Reassortment [J].

Furuse, Yuki ;

Suzuki, Akira ;

Kishi, Makiko ;

Nukiwa, Nao ;

Shimizu, Midori ;

Sawayama, Rumi ;

Fuji, Naoko ;

Oshitani, Hitoshi .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (02) :369-374

[9] Mixed Infection and the Genesis of Influenza Virus Diversity [J].

Ghedin, Elodie ;

Fitch, Adam ;

Boyne, Alex ;

Griesemer, Sara ;

DePasse, Jay ;

Bera, Jayati ;

Zhang, Xu ;

Halpin, Rebecca A. ;

Smit, Marita ;

Jennings, Lance ;

St George, Kirsten ;

Holmes, Edward C. ;

Spiro, David J. .

JOURNAL OF VIROLOGY, 2009, 83 (17) :8832-8841

[10]

Hall RJ, 2009, EUROSURVEILLANCE, V14, P10

← 1 2 →