Purification and applications of a phospholipase D from a new strain of Streptomyces

被引：17

作者：

Carrea, G

DArrigo, P

Secundo, F

Servi, S

机构：

[1] CNR,IST CHIM ORMONI,I-20131 MILAN,ITALY

[2] POLITECN MILAN,DIPARTIMENTO CHIM,CNR,CTR STUDIO SOSTANZE ORGAN NAT,I-20131 MILAN,ITALY

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 11期

关键词：

D O I：

10.1023/A:1018432325123

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

An enzyme with phospholipase D activity was purified to homogeneity from a new strain of Streptomyces. The molecular mass, assessed by electrospray mass spectrometry, was 52672 Da and the isoelectric point 9.2. The enzyme, which had pH optimum between 4 and 7, showed satisfactory stability and transphosphatidylation activity.

引用

页码：1083 / 1085

页数：3

共 12 条

[1]

PHOSPHOLIPIDS CHIRAL AT PHOSPHORUS - SYNTHESIS OF CHIRAL PHOSPHATIDYLCHOLINE AND STEREOCHEMISTRY OF PHOSPHOLIPASE-D [J].