Evaluation of gene polymorphisms in exercise-induced oxidative stress and damage

被引：37

作者：

Akimoto, Arthur Kenji ^{[1
]}

Miranda-Vilela, Ana Luisa ^{[1
]}

Zaidan Alves, Penha Cristina ^{[1
]}

Da Silva Pereira, Luiz Carlos ^{[2
]}

Lordelo, Graciana Souza ^{[1
]}

Hiragi, Cassia De Oliveira ^{[1
]}

Rodrigues Da Silva, Izabel Cristina ^{[1
]}

Grisolia, Cesar Koppe ^{[1
]}

Klautau-Guimaraes, Maria De Nazare ^{[1
]}

机构：

[1] Univ Brasilia, Inst Ciencias Biol, Dept Genet & Morfol, Genet Lab, BR-70910900 Brasilia, DF, Brazil

[2] Lab Sabin Anal Clin, Brasilia, DF, Brazil

来源：

FREE RADICAL RESEARCH | 2010年 / 44卷 / 03期

关键词：

Exercise-induced damage; biochemical markers of tissue injuries (AST; ALT; CK); TBARS assay; comet assay; antioxidant enzyme polymorphisms; POLYMERASE-CHAIN-REACTION; SUPEROXIDE-DISMUTASE; DNA-DAMAGE; CREATINE-KINASE; HAPTOGLOBIN POLYMORPHISM; SKELETAL-MUSCLE; FREE-RADICALS; CANCER RISK; SERUM; ASSOCIATION;

D O I：

10.3109/10715760903494176

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Many potentially significant genetic variants related to oxidative stress have been identified and performance in endurance sports is a multi-factorial phenotype. Thus, it was decided to investigate the influences of the haptoglobin (Hp), MnSOD (Val9Ala), CAT (21A/T), GPX1 (Pro198Leu), ACE, glutathione S-transferases M1 (GSTM1) and T1 (GSTT1) genes' polymorphisms on the oxidative stress and damage suffered by human athletes (runners). Blood samples taken immediately after a race were submitted to genotyping, comet and TBARS assays, biochemical analyses of creatine kinase (CK), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). MnSOD significantly influenced results of CK and a possible association between Hp1F-1S and Hp1S-2 genotypes with a superior TBARS values was found. Higher or lower TBARS and CK values or DNA damage also depended on the interaction between Hp and ACE or GST genotypes, indicating that MnSOD and Hp polymorphisms can be determining factors in performance, at least for runners.

引用

页码：322 / 331

页数：10

共 59 条

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