Chitosan enhances platelet adhesion and aggregation

被引：212

作者：

Chou, TC

Fu, E

Wu, CJ

Yeh, JH

机构：

[1] Natl Def Med Ctr, Dept Physiol, Taipei, Taiwan

[2] Natl Def Med Ctr, Tri Serv Gen Hosp, Sch Dent, Dept Periodontol, Taipei, Taiwan

[3] Natl Def Med Ctr, Inst Prevent Med, Taipei, Taiwan

[4] Kaohsiung Vet Gen Hosp, Div Nephrol, Kaohsiung, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 302卷 / 03期

关键词：

chitosan; platelet adhesion; aggregation; intracellular calcium level; glycoprotein IIb/IIIa;

D O I：

10.1016/S0006-291X(03)00173-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, chitosan (MW = 50,000) was tested for its enhancing platelet activity in rabbit platelet suspensions and the possible mechanisms involved were further investigated. Our results showed that after initial (5 min) and long-term (30 min) contact of platelets with chitosan, the platelet adhesion to chitosan-coated microtiter plates was dose-dependently increased compared to that of solvent control. Similarly, chitosan also dose-dependently increased the platelet aggregation and the intracellular free Ca2+ rise of Fura-2-AM loaded platelets. Additionally, in the presence of FITC-labeled anti-CD41/CD61, chitosan significantly enhanced the expression of platelet glycoprotein IIb/IIIa complex assayed by a flow cytometer. It is concluded that chitosan is an effective inducer for platelet adhesion and aggregation and the mechanisms of action of chitosan may be associated, at least partly, with the increasing [Ca2+](i) mobilization and enhancing expression of GPIIb/IIIa complex on platelet membrane surfaces. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：480 / 483

页数：4

共 25 条

[1] FLOW CYTOMETRIC MEASUREMENT OF PLATELET-FUNCTION AND RETICULATED PLATELETS [J].