Molecular structure of a novel gypsy-Ty3-like retrotransposon (Kabuki) and nested retrotransposable elements on the W chromosome of the silkworm Bombyx mori

被引:40
作者
Abe, H
Ohbayashi, F
Shimada, T
Sugasaki, T
Kawai, S
Mita, K
Oshiki, T
机构
[1] Tokyo Univ Agr & Technol, Fac Agr, Dept Biol Prod, Fuchu, Tokyo 1838509, Japan
[2] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
[3] Natl Inst Radiol Sci, Genome Res Grp, Inage Ku, Chiba 2638555, Japan
来源
MOLECULAR AND GENERAL GENETICS | 2000年 / 263卷 / 06期
基金
日本学术振兴会;
关键词
retrotransposon; gypsy-Ty3; Bombyx mori; W chromosome;
D O I
10.1007/s004380000270
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We previously characterized a female-specific randomly amplified polymorphic DNA (RAPD), designated W-Kabuki, derived from the W chromosome of the silkworm, Bombyx mori. To further analyze the W chromosome of B. mori, we obtained a lambda phage clone which contains the W-Kabuki RAPD sequence and sequenced the 18.1-kb DNA insert. We found that this DNA comprises a nested structure of at least seven elements; three retrotransposons, two retroposons, one functionally unknown insertion, and one Bombyx repetitive sequence. The non-LTR retrotransposon BMC1, the retroposon Bm1, a functionally unknown inserted DNA (FUI), and a copia-like LTR retrotransposon (Yokozuna) are themselves inserted into a novel gypsy-Ty3-like LTR retrotransposon, named Kabuki. Furthermore, this Kabuki element is itself inserted into another copy of Bm1. The BMC1. and Yolroz2mn elements inserted in the Kabuki sequence are intact. Moreover, the Kabuki element is largely intact. These results suggest that many retrotransposable elements have accumulated on the W chromosome, and these elements are expected to evolve more slowly than those on other chromosomes.
引用
收藏
页码:916 / 924
页数:9
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