Reduced cortical ecto-ATPase activity in rat brains during prolonged status epilepticus induced by sequential administration of lithium and pilocarpine

被引:21
作者
Nagy, AK [1 ]
Walton, NY [1 ]
Treiman, DM [1 ]
机构
[1] W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90095 USA
关键词
ecto-ATPase; synaptosomes; rat; lithium/pilecarpine; status epilepticus; subcellular brain fractions; Mg2+-dependent ATPase; Ca2+-dependent ATPase;
D O I
10.1007/BF02815238
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Considerable evidence indicates that ATP, acting intracellularly or as a neurotransmitter, can influence nerve cell physiology in a variety of ways. Defects in the functioning of ATP-metabolizing enzymes could therefore lead to disturbances in neurotransmission and creation of sustained neuronal discharges characteristic of status epilepticus. in this study we investigated synaptosomal ATPase changes in rat brains during lithium/pilocarpine-induced status epilepticus. After 2 h of continuous electroencephalographic spiking, both Mg2+- and Ca2+-dependent ecto-ATPases were significantly decreased in freshly prepared synaptosomal preparations from the status rats. The intracellulary acting Ca2+M2+-ATPase (Ca-pump) was also decreased, but no changes occurred in synaptosomal Na+K+-ATPase activity. The difference between ecto-ATPase activities of the control and status rat brains was not affected by repeated freezing-thawing and lengthy storage. Possible involvement of reduced synaptosomal divalent cation-dependent ATPases in the pathophysiology of status epilepticus is discussed.
引用
收藏
页码:135 / 147
页数:13
相关论文
共 52 条
[1]  
ALLEN RM, 1994, GENETICS, V137, P257
[2]  
Battastini AMO, 1995, BIOCHEM MOL BIOL INT, V37, P209
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]  
BUCHHALTER JR, 1993, EPILEPSIA S3, V34, pS3
[5]  
CHAUDRY IH, 1985, PURINES PHARM PHYSL, P115
[6]   SYNAPTIC PLASMA MEMBRANES FROM RAT BRAIN SYNAPTOSOMES - ISOLATION AND PARTIAL CHARACTERIZATION [J].
COTMAN, CW ;
MATTHEWS, DA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 249 (02) :380-+
[7]   ADENOSINE - A POTENTIAL MEDIATOR OF SEIZURE ARREST AND POSTICTAL REFRACTORINESS [J].
DURING, MJ ;
SPENCER, DD .
ANNALS OF NEUROLOGY, 1992, 32 (05) :618-624
[8]   ATP RECEPTOR-MEDIATED SYNAPTIC CURRENTS IN THE CENTRAL-NERVOUS-SYSTEM [J].
EDWARDS, FA ;
GIBB, AJ ;
COLQUHOUN, D .
NATURE, 1992, 359 (6391) :144-147
[9]  
ENGELHARDT WA, 1957, P INT S ENZ CHEM TOK, V2, P163
[10]   ATP MEDIATES FAST SYNAPTIC TRANSMISSION IN MAMMALIAN NEURONS [J].
EVANS, RJ ;
DERKACH, V ;
SURPRENANT, A .
NATURE, 1992, 357 (6378) :503-505