High temperature conjugation of proteins with carbohydrates

被引：48

作者：

Boratynski, J

Roy, R

机构：

[1] Polish Acad Sci, Ludwik Hirszfeld Inst Immunol & Expt Therapy, Dept Tumor Immunol, PL-53114 Wroclaw, Poland

[2] Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada

来源：

GLYCOCONJUGATE JOURNAL | 1998年 / 15卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

protein; conjugate; carbohydrate; lyophilization; vaccine; temperature; heating;

D O I：

10.1023/A:1007067513242

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new procedure was used to conjugate lactose and dextran with BSA without using coupling or activating reagents. The method is simple, rapid and cheap. Reducing sugars covalently bind to proteins when lyophilized together and briefly heated to a high temperature.

引用

页码：131 / 138

页数：8

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