A defined long-term in vitro tissue engineered model of neuromuscular junctions

被引：76

作者：

Das, Mainak ^{[1
]}

Rumsey, John W. ^{[1
]}

Bhargava, Neelima ^{[1
]}

Stancescu, Maria ^{[1
]}

Hickman, James J. ^{[1
]}

机构：

[1] Univ Cent Florida, Hybrid Syst Lab, NanoSci Technol Ctr, Orlando, FL 32826 USA

来源：

BIOMATERIALS | 2010年 / 31卷 / 18期

关键词：

In vitro test; Muscle; Nerve tissue engineering; Neural cell; Surface modification; Self assembly; SERUM-FREE MEDIUM; AMYLOID PRECURSOR PROTEIN; SKELETAL-MUSCLE CELLS; SPINAL-CORD NEURONS; SILICON MICROSTRUCTURES; ORGANOSILANE SURFACE; GROWTH-FACTORS; DIFFERENTIATION; PROMOTES; CULTURE;

D O I：

10.1016/j.biomaterials.2010.02.055

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Neuromuscular junction (NMJ) formation, occurring between motoneurons and skeletal muscle, is a complex multistep process involving a variety of signaling molecules and pathways. In vitro motoneuron-muscle co-cultures are powerful tools to study the role of different growth factors, hormones and cellular structures involved in NMJ formation. In this study, a serum-free culture system utilizing defined temporal growth factor application and a non-biological substrate resulted in the formation of robust NMJs. The system resulted in long-term survival of the co-culture and selective expression of neonatal myosin heavy chain, a marker of myotube maturation. NMJ formation was verified by colocalization of dense clusters of acetylcholine receptors visualized using alpha-bungarotoxin and synaptophysin containing vesicles present in motoneuron axonal terminals. This model will find applications in basic NMJ research and tissue engineering applications such as bio-hybrid device development for limb prosthesis and regenerative medicine as well as for high-throughput drug and toxin screening applications. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：4880 / 4888

页数：9

共 53 条

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