Role of chondroitin sulfate hyaluronan interactions in the viscoelastic properties of extracellular matrices and fluids

被引:57
作者
Nishimura, M
Yan, WQ
Mukudai, Y
Nakamura, S
Nakamasu, K
Kawata, M
Kawamoto, T
Noshiro, M
Hamada, T
Kato, Y [1 ]
机构
[1] Hiroshima Univ, Dept Biochem, Hiroshima 734, Japan
[2] Hiroshima Univ, Sch Dent, Dept Prosthet Dent, Hiroshima 734, Japan
[3] Hiroshima Univ, Dept Endodontol & Periodontol, Hiroshima 734, Japan
[4] Denki Kagaku Kogyo Co Ltd, Tokyo 194, Japan
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1998年 / 1380卷 / 01期
关键词
hyaluronan; chondroitin sulfate; viscosity; glycosaminoglycan;
D O I
10.1016/S0304-4165(97)00119-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The purpose of this study was to investigate the role of chondroitin sulfate-hyaluronan interactions in the viscoelastic properties of tissues and fluids, using capillary and cone-on-plate viscometers. Chondroitin sulfate markedly increased the viscosity of hyaluronan solutions at a wide range of hyaluronan mass (50-1900 kDa) under physiological conditions of pH, temperature, ionic strength and glycosaminoglycan concentration (0.5-40 mg/ml), although the viscosity of the chondroitin sulfate solutions themselves was very low. In the assay using a cone-on-plate viscometer, chondroitin sulfate increased the viscosity of hyaluronan solutions at various shear rates. At low shear rates, the viscosity of a chondroitin sulfate (5 mg/ml)-hyaluronan (0.5 mg/ml) mixture was about 40%?a of that of an aggrecan (5 mg/ml)-hyaluronan (0.5 mg/ml) mixture, and at 2.8-fold higher concentrations, chondroitin sulfate elicited the same effect on the viscosity of hyaluronan solutions (5 mg/ml) as an aggrecan monomer. In the presence of oscillatory motion, the addition of aggrecan increased the elasticity (storage) modulus G' and the viscosity (loss) modulus G " of hyaluronan solutions and markedly decreased the loss tangent G "/G' at frequencies corresponding to normal joint movements. In contrast, chondroitin sulfate had only a marginal effect on the loss tangent G "/G', although it increased G' and G ". These findings demonstrated that chondroitin sulfate, as well as aggrecan, increases the viscosity of hyaluronan solutions, although chondroitin sulfate has less effect on the elasticity of hyaluronan solutions than that of aggrecan, and suggest that chondroitin sulfate may play an important physiological role in determining the viscoelastic properties of extracellular matrices and fluids. (C) 1998 Elsevier Science B.V.
引用
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页码:1 / 9
页数:9
相关论文
共 49 条
[1]   Glycosylation of CD44 is implicated in CD44-mediated cell adhesion to hyaluronan [J].
Bartolazzi, A ;
Nocks, A ;
Aruffo, A ;
Spring, F ;
Stamenkovic, I .
JOURNAL OF CELL BIOLOGY, 1996, 132 (06) :1199-1208
[2]  
BERNFIELD MR, 1973, AM ZOOL, V13, P1067
[3]   COMPOSITION OF BOVINE CARTILAGE AND BONE [J].
CAMPO, RD ;
TOURTELL.CD .
BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 141 (03) :614-&
[4]  
CATERSON B, 1990, J CELL SCI, V97, P411
[5]  
Chabreek P., 1991, J APP POLYM SCI APP, V48, P233, DOI [10.1002/app.1991.070480019, DOI 10.1002/APP.1991.070480019]
[6]  
CHRISTNER JE, 1979, J BIOL CHEM, V254, P4624
[7]  
COOKE AF, 1978, BIORHEOLOGY, V15, P129
[8]  
ENGSTROMLAURENT A, 1989, CIBA F SYMP, V143, P233
[9]  
FORRESTER JV, 1980, IMMUNOLOGY, V40, P435
[10]   ACID GLYCOSAMINOGLYCANS IN PLASMA .2. FINDINGS IN RHEUMATOID-ARTHRITIS [J].
FRIMAN, C ;
JUVANI, M ;
SKRIFVARS, B .
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY, 1977, 6 (03) :177-182