Assessing cytotoxicity of boron nitride nanotubes: Interference with the MTT assay

被引：153

作者：

Ciofani, Gianni ^{[1
]}

Danti, Serena ^{[2
]}

D'Alessandro, Delfo ^{[2
]}

Moscato, Stefania ^{[3
]}

Menciassi, Arianna ^{[1
,4
]}

机构：

[1] Scuola Super Sant Anna, Smart Mat Lab, Ctr MicroBioRobot, Italian Inst Technol, I-56025 Pisa, Italy

[2] Cisanello Hosp, I-56124 Pisa, Italy

[3] Univ Pisa, Dept Human Morphol & Appl Biol, I-56126 Pisa, Italy

[4] Scuola Super Sant Anna, CRIM Lab, I-56025 Pisa, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 394卷 / 02期

关键词：

Boron nitride nanotubes; MTT assay; WST-1; assay; Cytocompatibility testing; Neuroblastoma; CARBON NANOTUBES; TOXICITY;

D O I：

10.1016/j.bbrc.2010.03.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thanks to a non-covalent wrapping with glycol-chitosan, highly biocompatible and highly concentrated dispersions of boron nitride nanotubes were obtained and tested on human neuroblastoma cells. A systematic investigation of the cytotoxicity of these nanovectors with several complementary qualitative and quantitative assays allowed a strong interference with the MU metabolic assay to be highlighted, similar to a phenomenon already observed for carbon nanotubes, that would wrongly suggest toxicity of boron nitride nanotubes. These results confirm the high complexity of these new nanomaterials, and the needing of extensive investigations on their exciting potential applications in the biomedical field. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：405 / 411

页数：7

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