Isolation and characterization of xylan-degrading alkali-tolerant thermophiles

被引：21

作者：

Dimitrov, PL ^{[1
]}

Kambourova, MS ^{[1
]}

Mandeva, RD ^{[1
]}

Emanuilova, EI ^{[1
]}

机构：

[1] Inst Microbiol, Sofia 1113, Bulgaria

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 157卷 / 01期

关键词：

thermophilic bacteria; alkali-tolerant bacteria; xylan-degrading enzyme;

D O I：

10.1111/j.1574-6968.1997.tb12748.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two alkali-tolerant thermophilic bacterial strains with xylanolytic activity were isolated by continuous cultivation from samples collected near Bulgarian hot springs. Xylanases produced by the two strains were similar with respect to temperature and pH optimum (70-75 degrees C and pH 6.5-7.0) as well as their thermostability. The xylanases were thermostable at 70 degrees C for 30 min. Xylanolytic activity was resistant to pH 5.5-8.0 (strain SP) and 6.0-7.5 (strain BC). Zymogram analysis of the culture supernatant ultrafiltrate showed two xylanase active bands corresponding to a protein band of 92 kDa.

引用

页码：27 / 30

页数：4

共 6 条

[1] CHARACTERIZATION OF CELLULASE-FREE XYLANASES FROM THE NEWLY ISOLATED BACILLUS SP STRAIN BP-23 [J].

BLANCO, A ;

PASTOR, FIJ .

CANADIAN JOURNAL OF MICROBIOLOGY, 1993, 39 (12) :1162-1166

[2] PURIFICATION AND PROPERTIES OF XYLANASE-A FROM ALKALI-TOLERANT BACILLUS SP STRAIN BP-23 [J].

BLANCO, A ;

VIDAL, T ;

COLOM, JF ;

PASTOR, FIJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (12) :4468-4470

[3] BACTERIAL CELLULASES AND XYLANASES [J].

GILBERT, HJ ;

HAZLEWOOD, GP .

JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 :187-194

[4] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[5] THERMOPHILIC ALKALINE XYLANASE FROM NEWLY ISOLATED ALKALIPHILIC AND THERMOPHILIC BACILLUS SP STRAIN TAR-1 [J].

NAKAMURA, S ;

NAKAI, R ;

WAKABAYASHI, K ;

ISHIGURO, Y ;

AONO, R ;

HORIKOSHI, K .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (01) :78-81

[6] MULTIPLICITY OF BETA-1,4-XYLANASE IN MICROORGANISMS - FUNCTIONS AND APPLICATIONS [J].

WONG, KKY ;

TAN, LUL ;

SADDLER, JN .

MICROBIOLOGICAL REVIEWS, 1988, 52 (03) :305-317

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