Glutathione-bound gold nanoclusters for selective-binding and detection of glutathione S-transferase-fusion proteins from cell lysates

被引:101
作者
Chen, Cheng-Tai [1 ]
Chen, Wei-Jen [1 ]
Liu, Chao-Zong [2 ,3 ]
Chang, Ling-Ya [2 ,3 ]
Chen, Yu-Chie [1 ]
机构
[1] Natl Chiao Tung Univ, Dept Appl Chem, Hsinchu 300, Taiwan
[2] Tzu Chi Univ, Dept Pharmacol, Hualien 970, Taiwan
[3] Tzu Chi Univ, Inst Pharmacol & Toxicol, Hualien 970, Taiwan
关键词
CLUMPING-FACTOR-A; CORE/SHELL NANOPARTICLES; MAGNETIC NANOPARTICLES; CONCENTRATING PROBES; ESCHERICHIA-COLI; RAPID ENRICHMENT; PHOSPHOPEPTIDES; FIBRINOGEN; PURIFICATION; MOLECULES;
D O I
10.1039/b916919a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A straightforward method for the rapid detection of the presence of glutathione S-transferase (GST)-tagged proteins from sample solutions using glutathione (GSH)-bound gold nanoclusters (Au@GSH NCs) with luminescence properties as the detection probes by simple observation with the naked eye was proposed in this study.
引用
收藏
页码:7515 / 7517
页数:3
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