Engrailed-1 misexpression in chick embryos prevents apical ridge formation but preserves segregation of dorsal and ventral ectodermal compartments

被引:15
作者
Altabef, M
Logan, C
Tickle, C
Lumsden, A
机构
[1] Guys Hosp, Univ London Kings Coll, Dept Dev Neurobiol, London SE1 9RT, England
[2] UCL, Dept Anat & Dev Biol, London WC1E 6BT, England
基金
英国医学研究理事会; 英国惠康基金;
关键词
En-1; selector gene; compartment; limb development; rhodamine dextran;
D O I
10.1006/dbio.2000.9659
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Using lineage tracers, we recently showed dorsal and ventral ectodermal compartments along the sides of the body in chick embryos. The compartments are formed both in presumptive limb-forming regions where they position the apical ridge and also in presumptive interlimb (flank). Here we show, using a novel technique combining fate mapping and in situ hybridisation, that the ventral compartment coincides with the Engrailed-1 (En-1) domain of expression. This coincidence suggests that En-l could maintain the ventral compartment and be necessary for apical ridge formation. To test this hypothesis, we ectopically expressed En-1 via retroviral transfer and then examined limb development and cell lineage restriction in the ectoderm. En-1 misexpression can completely prevent formation of both normal limbs and ectopic limbs induced in the flank by application of FGF-2. In both cases, there are no morphological signs of apical ectodermal ridge formation and expression of ridge-associated genes is undetectable. In striking contrast, the lineage restriction between dorsal and ventral ectoderm is not altered. Therefore, En-1 is involved in the regulation of ridge formation but not compartment maintenance. (C) 2000 Academic Press.
引用
收藏
页码:307 / 316
页数:10
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